Greiser-Wilke I, Moennig V
Institute for Virology, Hannover Veterinary School, FRG.
Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):549-60. doi: 10.1016/0769-2609(87)90040-8.
Four mouse monoclonal antibodies generated against Brucella melitensis 16M, and three generated against B. suis 1330 were analysed. An ELISA (enzyme-linked-immunosorbent assay) with whole cells as antigens was used to determine cross-reactivities with other Gram-negative bacteria. Two antibodies showed cross-reactivity with smooth Brucella strains only. Five antibodies also reacted with Yersinia enterocolitica O:9, but not with other bacteria. Two of these antibodies had significantly higher titres with A greater than M serotype Brucella strains, indicating that these epitopes are related to the antigenic A complex. The antigenic determinants recognized by the monoclonal antibodies showed varying degrees of susceptibility towards oxidation. They were shown by immunoblotting to be located on the polysaccharide moiety of the O-side chain.
分析了针对羊种布鲁氏菌16M产生的4种小鼠单克隆抗体以及针对猪种布鲁氏菌1330产生的3种单克隆抗体。采用以全细胞为抗原的酶联免疫吸附测定（ELISA）来确定与其他革兰氏阴性菌的交叉反应性。两种抗体仅与光滑型布鲁氏菌菌株有交叉反应。5种抗体也与小肠结肠炎耶尔森氏菌O:9发生反应，但与其他细菌无反应。其中两种抗体对A血清型大于M血清型的布鲁氏菌菌株具有显著更高的效价，表明这些表位与A抗原复合物相关。单克隆抗体识别的抗原决定簇对氧化表现出不同程度的敏感性。免疫印迹显示它们位于O侧链的多糖部分。
