Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Immunology, Harvard Medical School, Boston, Massachusetts, USA.
mBio. 2020 Mar 17;11(2):e00191-20. doi: 10.1128/mBio.00191-20.
While there is no effective vaccine against infection, previous work has demonstrated the importance of -specific CD4 T cells (NR1 T cells) in pathogen clearance. Specifically, NR1 T cells have been shown to be protective in mice, and this protection depends on the host's ability to sense the cytokine gamma interferon (IFN-γ). However, it is unclear what role NR1 production or sensing of IFN-γ plays in T cell homing to the genital tract or T cell-mediated protection against Using two-photon microscopy and flow cytometry, we found that naive wild-type (WT), IFN-γ, and IFN-γR NR1 T cells specifically home to sections in the genital tract that contain We also determined that protection against infection requires production of IFN-γ from either NR1 T cells or endogenous cells, further highlighting the importance of IFN-γ in clearing infection. is an important mucosal pathogen that is the leading cause of sexually transmitted bacterial infections in the United States. Despite this, there is no vaccine currently available. In order to develop such a vaccine, it is necessary to understand the components of the immune response that can lead to protection against this pathogen. It is well known that antigen-specific CD4 T cells are critical for clearance, but the contexts in which they are protective or not protective are unknown. Here, we aimed to characterize the importance of gamma interferon production and sensing by T cells and the effects on the immune response to Our work here helps to define the contexts in which antigen-specific T cells can be protective, which is critical to our ability to design an effective and protective vaccine against .
虽然目前尚无针对 感染的有效疫苗，但之前的研究已经证明了 -特异性 CD4 T 细胞（NR1 T 细胞）在清除病原体方面的重要性。具体而言，已经证明 NR1 T 细胞在小鼠中具有保护作用，这种保护作用取决于宿主感知细胞因子γ干扰素（IFN-γ）的能力。然而，NR1 的产生或 IFN-γ的感知在 T 细胞归巢到生殖道或 T 细胞介导的针对 感染的保护中所起的作用尚不清楚。我们使用双光子显微镜和流式细胞术发现，幼稚的野生型（WT）、IFN-γ 和 IFN-γR NR1 T 细胞特异性归巢到生殖道中包含 的区域。我们还确定，针对感染的保护需要来自 NR1 T 细胞或内源性细胞的 IFN-γ产生，这进一步强调了 IFN-γ在清除 感染中的重要性。是一种重要的黏膜病原体，是美国性传播细菌感染的主要原因。尽管如此，目前尚无可用的疫苗。为了开发这种疫苗，有必要了解可以导致针对这种病原体的保护的免疫反应的组成部分。众所周知，抗原特异性 CD4 T 细胞对于 清除至关重要，但它们在何种情况下具有保护作用或无保护作用尚不清楚。在这里，我们旨在表征 T 细胞产生和感知γ干扰素的重要性以及对免疫反应的影响。我们的工作有助于定义抗原特异性 T 细胞可以具有保护作用的情况，这对于我们设计针对 的有效和保护性疫苗的能力至关重要。
