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长链非编码RNA LINC01140通过调控miR-199a-3p/ZHX1轴抑制胶质瘤细胞的迁移和侵袭。

LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis.

作者信息

Xin Yanchao, Zhang Wuzhong, Mao Chongchong, Li Jianxin, Liu Xianzhi, Zhao Junbo, Xue Junfeng, Li Junqing, Ren Yonglu

机构信息

Department of Neurosurgery, People's Hospital of Jiaozuo City, Jiaozuo City, Henan Province 454002, People's Republic of China.

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University in Henan Province, Zhengzhou City, Henan Province 450052, People's Republic of China.

出版信息

Onco Targets Ther. 2020 Feb 28;13:1833-1844. doi: 10.2147/OTT.S230895. eCollection 2020.

DOI:10.2147/OTT.S230895
PMID:32184618
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7053821/
Abstract

PURPOSE

Glioma is an aggressive tumor from the nervous system, which causes more than 70% of primary malignant brain tumors. Considering its severe malignancy, there is an urgent need to investigate more practical markers to understand the pathogenesis of glioma, and potential treatment methods for glioma patients. In the paper, we are focused on examining the roles of LINC01140, miR-199a-3p, and ZHX1 in the progression of gliomas, as well as their inner associations and modulation mechanisms.

METHODS

qRT-PCR was employed to examine the expression levels of LINC01140 and miR-199a-3p. We measured the expressions of ZHX1 via qRT-PCR and Western blotting. CCK8 assays, migration assays, and invasion assays were carried out to determine the cell viabilities and abilities of migration and invasion. We also conducted in vivo tumor growth experiments to investigate the roles of LINC01140 in glioma developments.

RESULTS

The expressions of LINC01140 were promoted in glioma. Silencing LINC01140 could inhibit glioma cell viabilities, migration, and invasion. In our experiments, miR-199a-3p was inhibited in glioma. LINC01140 negatively regulated the expressions of miR-199a-3p in glioma. MiR-199a-3p could target ZHX1 to inhibit its expression in glioma cells.

CONCLUSION

LINC01140 could promote glioma developments by modulating the miR-199a-3p/ZHX1 axis.

摘要

目的

胶质瘤是一种侵袭性神经系统肿瘤，占原发性恶性脑肿瘤的70%以上。鉴于其严重的恶性程度，迫切需要研究更实用的标志物以了解胶质瘤的发病机制以及针对胶质瘤患者的潜在治疗方法。在本文中，我们着重研究LINC01140、miR-199a-3p和ZHX1在胶质瘤进展中的作用，以及它们之间的内在联系和调控机制。

方法

采用qRT-PCR检测LINC01140和miR-199a-3p的表达水平。通过qRT-PCR和蛋白质免疫印迹法检测ZHX1的表达。进行CCK8实验、迁移实验和侵袭实验以确定细胞活力、迁移和侵袭能力。我们还进行了体内肿瘤生长实验以研究LINC01140在胶质瘤发展中的作用。

结果

胶质瘤中LINC01140的表达升高。沉默LINC01140可抑制胶质瘤细胞活力、迁移和侵袭。在我们的实验中，胶质瘤中miR-199a-3p受到抑制。LINC01140在胶质瘤中负向调节miR-199a-3p的表达。miR-199a-3p可靶向ZHX1以抑制其在胶质瘤细胞中的表达。

结论

LINC01140可通过调节miR-199a-3p/ZHX1轴促进胶质瘤发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/3519de270ead/OTT-13-1833-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/b350708288e8/OTT-13-1833-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/cdf5594170c2/OTT-13-1833-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/457684b941d1/OTT-13-1833-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/3c2ff19a340e/OTT-13-1833-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/cb57173548d2/OTT-13-1833-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/2bed08584c0a/OTT-13-1833-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/3519de270ead/OTT-13-1833-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/b350708288e8/OTT-13-1833-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/cdf5594170c2/OTT-13-1833-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/457684b941d1/OTT-13-1833-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/3c2ff19a340e/OTT-13-1833-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/cb57173548d2/OTT-13-1833-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/2bed08584c0a/OTT-13-1833-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789a/7053821/3519de270ead/OTT-13-1833-g0007.jpg

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Downregulation of LINC01140 is associated with adverse features of breast cancer.LINC01140的下调与乳腺癌的不良特征相关。
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LINC01140 promotes the progression and tumor immune escape in lung cancer by sponging multiple microRNAs.LINC01140通过吸附多种微小RNA促进肺癌进展和肿瘤免疫逃逸。
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MicroRNA-199a-3p inhibits tumorigenesis of hepatocellular carcinoma cells by targeting ZHX1/PUMA signal.微小RNA-199a-3p通过靶向ZHX1/PUMA信号抑制肝癌细胞的肿瘤发生。
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A three-lncRNA expression signature associated with the prognosis of gastric cancer patients.与胃癌患者预后相关的三个长链非编码 RNA 表达特征。
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