Chen Shuangshuang, Yu Miao, Li He, Wang Ying, Lu Zhuchou, Zhang Yunxing, Liu Mingying, Qiao Guirong, Wu Longhua, Han Xiaojiao, Zhuo Renying
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.
Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China.
Front Plant Sci. 2020 Feb 28;11:142. doi: 10.3389/fpls.2020.00142. eCollection 2020.
The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from . Designated as , the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of under Cd stress was characterized in transgenic and non-hyperaccumulation ecotype . was able to rescue the Cd sensitivity of the mutant. reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and in transgenic lines. The present results suggest that increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of .
热激转录因子（Hsf）家族是植物应激反应中的重要成员，影响植物对镉（Cd）的耐受性。在本研究中，我们从……鉴定并对Hsf A4亚组的一个转录本进行了功能表征。该转录本命名为……，开放阅读框长1302 bp，编码一个推定的含433个氨基酸的蛋白质，其中包含一个完整的DNA结合结构域（DBD）。……在酵母中的异源表达增强了对Cd胁迫的耐受性和积累，而包含一个内含子且具有不完整DBD的可变剪接转录本……的表达，导致转基因酵母中对Cd胁迫的耐受性相对较差且Cd积累量较低。在转基因……和非超积累生态型……中对……在Cd胁迫下的功能进行了表征。……能够挽救……突变体对Cd的敏感性。……减少了转基因系中活性氧（ROS）的积累，并增加了ROS清除酶基因和……的表达。目前的结果表明，……通过上调ROS清除酶的活性和……的表达来增强植物对胁迫的抗性。
