Emami Seyed Ahmad, Kheshami Shima, Ramazani Elham, Akaberi Maryam, Iranshahy Milad, Kazemi Seyed Mahammad, Tayarani-Najaran Zahra
Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Iran J Pharm Res. 2019 Fall;18(4):1912-1920. doi: 10.22037/ijpr.2019.14665.12599.
Chaharmahal and Bakhtiari Province is one of the most important endemism states of the flora of Iran with a considerable plant species diversity. In the present study, the cytotoxic activity of 13 plant species grown in Chaharmahal and Bakhtiari have been evaluated on prostate (PC-3), breast (MCF-7), liver (HepG2), ovary (CHO), and melanoma (B16-F10) cancer cell lines. The cytotoxicity and apoptotic activity of methanol extracts was evaluated using resazurin reagent and flow cytometry of PI stained cells, respectively. Methanol extracts of , and on CHO cell line ( <0.05) and and on B16/F10 cell line ( <0.05) showed significant cytotoxic effects and increased apoptosis. It is generally suggested that the plant extracts with low IC values are likely to be used as anti-cancer compounds in reducing cancer progression in scientific studies.
查哈马哈勒-巴赫蒂亚里省是伊朗植物区系最重要的特有地区之一，拥有相当丰富的植物物种多样性。在本研究中，对生长在查哈马哈勒-巴赫蒂亚里省的13种植物对前列腺癌（PC-3）、乳腺癌（MCF-7）、肝癌（HepG2）、卵巢癌（CHO）和黑色素瘤（B16-F10）癌细胞系的细胞毒性活性进行了评估。分别使用刃天青试剂和PI染色细胞的流式细胞术评估甲醇提取物的细胞毒性和凋亡活性。[此处原文缺失植物名称]的甲醇提取物对CHO细胞系（P<0.05）以及[此处原文缺失植物名称]的甲醇提取物对B16/F10细胞系（P<0.05）显示出显著的细胞毒性作用并增加了细胞凋亡。一般认为，在科学研究中，具有低半数抑制浓度值的植物提取物有可能被用作抗癌化合物以减少癌症进展。
