Jassbi Amir Reza, Miri Ramin, Asadollahi Mojtaba, Javanmardi Najmeh, Firuzi Omidreza
Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
Pharm Biol. 2014 Jan;52(1):62-7. doi: 10.3109/13880209.2013.810650. Epub 2013 Sep 12.
Woundwort (Stachys) plants from the Lamiaceae family have been used in folk medicine for various purposes.
This study was designed to analyze cytotoxic, antioxidant and antimicrobial activities of Stachys plants, because these fields have extensively benefited of drug discovery from natural sources.
Nine Stachys plants were collected from different regions of Iran. Cytotoxic activities of methanol, 80% methanol and dichloromethane (DCM) extracts of these plants were assessed on three human cancer cell lines (HL-60, K562 and MCF-7 cells) with the MTT assay, while antioxidant and antimicrobial activities were determined on methanol extracts by DPPH and nutrient broth micro-dilution assays, respectively.
DCM extract of St. pilifera Benth. had the lowest IC₅₀ in three cancer cell lines ranging from 33.1 to 48.2 µg/ml, followed by the 80% methanol extract of St. persica S.G.Gmel. ex C.A.Mey. (IC₅₀ range: 62.1-104.1 µg/ml) and DCM extract of St. byzantina C. Koch (IC50 range: 62.7-131.0 µg/ml). St. byzantina. St. lavandulifolia Vahl., St. acerosa Boiss., St. obtusicrena Boiss. and St. persica showed lowest IC₅₀ values in the DPPH scavenging assay (135.1, 162.6, 164.7, 169.4 and 172.4 µg/ml, respectively), while their total phenolic contents were 23.9, 18.2, 18.6, 20.4, 27.8 mg equivalent of gallic acid in 1 g dry plant, respectively. The methanol extracts of St. byzantina and St. persica inhibited all six tested Gram-negative and Gram-positive bacterial strains.
Various Stachys species (especially St. byzantina and St. persica) are valuable sources of natural compounds with important biological properties.
唇形科水苏属植物在民间医学中有多种用途。
本研究旨在分析水苏属植物的细胞毒性、抗氧化和抗菌活性，因为这些领域已从天然来源的药物发现中广泛受益。
从伊朗不同地区采集了9种水苏属植物。用MTT法评估这些植物的甲醇、80%甲醇和二氯甲烷（DCM）提取物对三种人类癌细胞系（HL-60、K562和MCF-7细胞）的细胞毒性，同时分别用DPPH法和营养肉汤微量稀释法测定甲醇提取物的抗氧化和抗菌活性。
绒毛水苏的DCM提取物在三种癌细胞系中的IC₅₀最低，范围为33.1至48.2μg/ml，其次是波斯水苏的80%甲醇提取物（IC₅₀范围：62.1 - 104.1μg/ml）和绵毛水苏的DCM提取物（IC₅₀范围：62.7 - 131.0μg/ml）。绵毛水苏、薰衣草叶水苏、尖齿水苏、钝裂水苏和波斯水苏在DPPH清除试验中显示出最低的IC₅₀值（分别为135.1、162.6、164.7、169.4和172.4μg/ml），而它们的总酚含量分别为每1g干植物中23.9、18.2、18.6、20.4、27.8mg没食子酸当量。绵毛水苏和波斯水苏的甲醇提取物对所有六种测试的革兰氏阴性和革兰氏阳性细菌菌株均有抑制作用。
各种水苏属植物（尤其是绵毛水苏和波斯水苏）是具有重要生物学特性的天然化合物的宝贵来源。
