毒理学分析中用于酸性药物常规筛查的薄层色谱系统的选择。

Choice of thin-layer chromatographic systems for the routine screening for acidic drugs during toxicological analyses.

作者信息

Owen P, Pendlebury A, Moffat A C

出版信息

J Chromatogr. 1978 Nov 21;161:195-203. doi: 10.1016/s0021-9673(01)85227-4.

Abstract

The efficiencies of fifteen thin-layer silica gel chromatographic systems in separating commonly encountered acidic drugs are compared. The discriminating powers of the systems are measured both individually and in combination. Ethyl acetate and chloroform-methanol (9:1) are found to be the two best systems. The combination of the ethyl acetate-methanol-ammonia (85:10:5) system with either of these gives the best pair of systems. Various sequences of spray reagents are also examined.

摘要

比较了15种薄层硅胶色谱系统分离常见酸性药物的效率。分别测定了这些系统以及它们组合后的鉴别能力。发现乙酸乙酯和氯仿 - 甲醇（9:1）是两种最佳系统。乙酸乙酯 - 甲醇 - 氨水（85:10:5）系统与这两种系统中的任何一种组合，都能得到最佳的系统对。还研究了各种喷雾试剂的顺序。