Physical Chemistry Department. Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.
Physical Chemistry Department. Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.
J Chromatogr A. 2020 Aug 16;1625:461277. doi: 10.1016/j.chroma.2020.461277. Epub 2020 May 30.
The influence of impregnation the chromatographic plate adsorbent layer, silica, with hen's egg white albumin (OVA) or bovine serum albumin (BSA) on the retention of some popular medicines (paracetamol, aminophenazone, theophylline, caffeine, acetanilide, ciprofloxacin, tramadol, acetylsalicylic acid, acebutolol) is investigated. The effect of composition and buffer pH of the mobile phase on solute separation selectivity is also studied. The chromatographic systems with and without above mentioned albumins and their influence on investigated drug retention are compared. In general, it has been turned out that retention of tested medicines in systems with the sorbent impregnated with albumin significantly increase relative to those with non-impregnated.
研究了将鸡蛋白白蛋白（OVA）或牛血清白蛋白（BSA）浸渍到色谱板吸附层硅胶上对某些常用药物（对乙酰氨基酚、氨基比林、茶碱、咖啡因、乙酰苯胺、环丙沙星、曲马多、阿司匹林、醋丁洛尔）保留的影响。还研究了流动相组成和缓冲 pH 值对溶质分离选择性的影响。比较了有和没有上述白蛋白的色谱系统及其对被研究药物保留的影响。一般来说，已经证明，与未浸渍的系统相比，用浸渍白蛋白的吸附剂填充的系统中测试药物的保留显著增加。
