Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
Microbiology & Fermentation Technology Department, Central Food Technological Research Institute, Mysore, 570020, India.
J Fluoresc. 2020 May;30(3):537-545. doi: 10.1007/s10895-020-02509-y. Epub 2020 Mar 17.
Interest in biosensing platforms using protein fluorescent gold nanoclusters (FGNCs) has grown significantly in the past due to the unique optical properties they offer. This study investigates the interaction of metal ions with FGNCs, and the structural modifications brought about by the interaction resulting in fluorescence changes of the cluster and its successful application in the detection of two heavy metals, cobalt and cadmium. The binding of cobalt and cadmium to FGNCs synthesized from BSA significantly altered the secondary structure of the protein, causing a change in its hydrophobicity. It also resulted in a change in fluorescence properties of FGNCs by intersystem crossing (ICT) and fluorescence resonance energy transfer (FRET). Cobalt and cadmium could successfully be detected in the range of 5-165 ng/mL (R = 0.95) and 20-1000 ng/ mL (R = 0.91), respectively, with appreciable sensitivity. The principle was also applied for the detection of Vitamin B in commercially available ampoules, validating the proposed method. Graphical Abstract Proposed detection method of cadmium and cobalt using FGNCs.
由于其独特的光学性质，使用蛋白质荧光金纳米簇 (FGNC) 的生物传感平台在过去引起了极大的兴趣。本研究调查了金属离子与 FGNC 的相互作用，以及相互作用引起的结构修饰导致簇的荧光变化及其在检测两种重金属钴和镉中的成功应用。钴和镉与从 BSA 合成的 FGNC 的结合显著改变了蛋白质的二级结构，导致其疏水性发生变化。它还通过系间穿越 (ICT) 和荧光共振能量转移 (FRET) 导致 FGNC 的荧光性质发生变化。钴和镉可以分别在 5-165 ng/mL（R=0.95）和 20-1000 ng/mL（R=0.91）的范围内成功检测到，具有可观的灵敏度。该原理还应用于市售安瓿中维生素 B 的检测，验证了所提出的方法。
