Department of Neurosurgery and Brain Repair, University of South Florida Morsani College of Medicine, Tampa, Florida, USA.
Section of Histology and Embryology, Department of Biomedicine, Neurosciences and Advanced Diagnostics (BIND), University of Palermo, Palermo, Italy.
Stem Cell Rev Rep. 2020 Jun;16(3):585-595. doi: 10.1007/s12015-020-09967-8.
Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a pivotal source of therapeutically active cells for regenerative medicine due to their multipotent differentiation potential, immunomodulatory and anti-inflammatory proprieties, as well as logistical collection advantages without ethical concerns. However, it remains poorly understood whether MSCs from different compartments of the human umbilical cord are therapeutically superior than others. In this study, MSCs were isolated from Wharton's jelly (WJ-MSCs), perivascular region (PV-MSCs) and cord lining (CL-MSCs) of hUC. These cells expressed the mesenchymal markers (CD90, CD73), stemness marker (OCT4), endothelial cell adhesion molecular marker (CD146), and the monocyte/macrophage marker (CD14) found within the MSC population implicated as a key regulator of inflammatory responses to hypoxia, was displayed by WJ-, PV-, and CL-MSCs respectively. A direct consequence of oxygen and glucose deprivation during stroke and reperfusion is impaired mitochondrial function that contributes to cellular death. Emerging findings of mitochondria transfer provide the basis for the replenishment of healthy mitochondria as a strategy for the treatment of stroke. Cell Energy Phenotype and Mito Stress tests were performed the energy metabolic profile of the three MSC populations and their mitochondrial function in both ambient and OGD cell culture conditions. PV-MSCs showed the highest mitochondrial activity. CL-MSCs were the least affected by OGD/R condition, suggesting their robust survival in ischemic environment. In this study, MSC populations in UC possess comparable metabolic capacities and good survival under normal and hypoxic conditions suggesting their potential as transplantable cells for mitochondrial-based stem cell therapy in stroke and other ischemic diseases.
人脐带间充质干细胞（hUC-MSCs）由于其多能分化潜能、免疫调节和抗炎特性以及在没有伦理问题的情况下逻辑上易于采集的优势，是再生医学中治疗活性细胞的重要来源。然而，人们对不同人脐带部位的间充质干细胞是否在治疗上优于其他细胞仍知之甚少。在这项研究中，我们从华通氏胶（WJ-MSCs）、血管周围区域（PV-MSCs）和脐带衬里（CL-MSCs）中分离出 MSC。这些细胞表达间充质标记物（CD90、CD73）、干细胞标记物（OCT4）、内皮细胞黏附分子标记物（CD146）和单核细胞/巨噬细胞标记物（CD14），这些标记物被认为是调节缺氧引起的炎症反应的关键调节剂，分别在 WJ-MSCs、PV-MSCs 和 CL-MSCs 中表达。在中风和再灌注期间，氧气和葡萄糖的剥夺直接导致线粒体功能受损，这导致细胞死亡。线粒体转移的新发现为补充健康的线粒体提供了基础，作为治疗中风的一种策略。在三种 MSC 群体的细胞能量表型和线粒体功能的正常和 OGD 细胞培养条件下进行了细胞能量表型和线粒体应激测试。PV-MSCs 显示出最高的线粒体活性。CL-MSCs 受 OGD/R 条件的影响最小，这表明它们在缺血环境中具有较强的生存能力。在这项研究中，UC 中的 MSC 群体具有相似的代谢能力，并且在正常和缺氧条件下具有良好的生存能力，这表明它们作为基于线粒体的干细胞治疗中风和其他缺血性疾病的移植细胞具有潜力。
