Department of Thoracic Surgery, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan, China.
Department of Pharmaceutical Sciences, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan, China.
J Clin Lab Anal. 2020 Aug;34(8):e23308. doi: 10.1002/jcla.23308. Epub 2020 Mar 17.
Recent reports have indicated that circular RNA (circRNA) may regulate tumorigenesis development. However, the function of circRNAs in esophageal squamous cell carcinoma (ESCC) is unclear.
The RT-qPCR assay was performed to detect hsa_circ_0012563 expression in ESCC tissues and cell lines. Then, the MTT assay, colony formation assay, flow cytometric assay, and cell migration and invasion assay were performed to examine the function of hsa_circ_0012563. In addition, the RT-PCR and Western blot were used to detect XRCC1 and epithelial-to-mesenchymal transition (EMT) related gene expression.
The RT-qPCR revealed that the hsa_circ_0012563 expression was remarkably upregulated in ESCC tissue and ESCC cell lines. Functionally, downregulation of hsa_circ_0012563 suppressed cell proliferation, migration, and invasion and promoted cell apoptosis. Mechanically, the knockdown of hsa_circ_0012563 inhibited XRCC1-mediated EMT pathway to suppress cell migration and invasion.
Therefore, these results reveal hsa_circ_0012563 is a critical oncogene and may be a novel biomarker in ESCC.
最近的报告表明，环状 RNA（circRNA）可能调节肿瘤发生发展。然而，circRNAs 在食管鳞状细胞癌（ESCC）中的功能尚不清楚。
采用 RT-qPCR 法检测 ESCC 组织和细胞系中 hsa_circ_0012563 的表达。然后，采用 MTT 法、集落形成实验、流式细胞术和细胞迁移侵袭实验检测 hsa_circ_0012563 的功能。此外，采用 RT-PCR 和 Western blot 检测 XRCC1 和上皮间质转化（EMT）相关基因的表达。
RT-qPCR 显示，hsa_circ_0012563 在 ESCC 组织和 ESCC 细胞系中表达显著上调。功能上，下调 hsa_circ_0012563 抑制细胞增殖、迁移和侵袭，促进细胞凋亡。机制上，hsa_circ_0012563 的敲低抑制了 XRCC1 介导的 EMT 通路，从而抑制了细胞的迁移和侵袭。
因此，这些结果表明 hsa_circ_0012563 是一种关键的癌基因，可能是 ESCC 的一种新型生物标志物。
