Department of Traditional Chinese Medicine, Beijing Children' s Hospital, Capital Medical University, National Center for Children's health, Beijing 100045, China.
J Tradit Chin Med. 2019 Aug;39(4):516-523.
To investigate the effect of the clinical effective prescription of Qingre Lishi Yishen decoction (QRLS) on the activation of mesangial cells in immunoglobulin A nephropathy (IgAN) rats.
IgAN rat's model was established by combine with intragastric administration of bovine serum albumin (BSA) + intravenous injection of lipopolysaccharide (LPS) by + subcutaneous injection of carbon tetrachloride (CCL4). Then the animals were randomly divided into four groups: control group, IgAN model group, IgAN model with Valsartan (Val) treatment group and IgAN model with QRLS treatment group. To observe the indexes of 24-h urine protein, renal function, deposition of immune complexes, expression of activation factor, fibrosis marker and inflammatory cytokines in four different groups.
The Val or QRLS treatment group: (a) it reduced the immune complexes deposition of IgA in glomerular mesangial and inhibited mesangial cell proliferation; (b) it decreased the expression of smooth muscle actin (α-SMA), fibronectin (FN) and tumor necrosis factor alpha (TNF-α).
The study suggested that QRLS ameliorate renal structure and function in IgAN rat's model. Furthermore, we also observed that QRLS alleviated mesangial cells activation and matrix accumulation partly by decreasing the α-SMA, then to downregu.
探讨清热利湿益肾方对免疫球蛋白 A 肾病（IgAN）大鼠系膜细胞活化的影响。
采用牛血清白蛋白（BSA）灌胃+脂多糖（LPS）尾静脉注射+四氯化碳（CCL4）皮下注射复合方法建立 IgAN 大鼠模型，将动物随机分为 4 组：对照组、IgAN 模型组、IgAN 模型缬沙坦（Val）治疗组和 IgAN 模型清热利湿益肾方治疗组。观察各组大鼠 24 小时尿蛋白、肾功能、免疫复合物沉积、活化因子、纤维化标志物及炎症细胞因子的指标。
缬沙坦或清热利湿益肾方治疗组：（a）减少肾小球系膜区 IgA 免疫复合物沉积，抑制系膜细胞增殖；（b）降低平滑肌肌动蛋白（α-SMA）、纤维连接蛋白（FN）和肿瘤坏死因子-α（TNF-α）的表达。
本研究表明，清热利湿益肾方可改善 IgAN 大鼠的肾脏结构和功能。此外，我们还观察到，清热利湿益肾方通过减少 α-SMA 来减轻系膜细胞的活化和基质积聚，从而下调。
