Zhang Ying-Ying, Liu Qing-Guo, Xu Meng, Guo Yan, Liu Jin-Yan
Zhongguo Zhen Jiu. 2014 Aug;34(8):791-7.
To explore the effect differences between twirling-rotating reinforcing and reducing technique of acupuncture on cardiac damage in spontaneous hypertensive rats (SHR).
Sixty male 11-week-old SHR were randomly divided into four groups: a model control group (group A), a twirling-rotating reinforcing technique group (group B), a twirling-rotating reducing technique group (group C) and a needle retaining group (group D), 15 rats in each one. In addition, twelve male 11-week-old Wistar rats were used as a blank control group (group E). Acupuncture was not used in group A and group E, only with grasp, capture and binding stimulation that was also adapted in the rest groups. Rats in the group B were treated with acupuncture at "Taichong" (LR 3) by twirling-rotating reinforcing technique for 1 min and then the needles were retained for 9 min; rats in the group C were treated with acupuncture at "Taichong" (LR 3) by twirling-rotating reducing technique for 1 min and then the needles were retained for 9 min; rats in the group D were treated with acupuncture at "Taichong" (LR 3) but without any technique and then needles were retained for 10 min. Before and after acupuncture, blood pressure monitor was used to measure the rats' systolic pressure and diastolic pressure every 6 days. Twenty-eight days after the treatment, HE and Masson staining were adopted to observe the status of left ventricular hypertrophy and myocardial fibrosis. ELISA method was applied to test the content of endothelin-1 (ET-1). PCR semiquantitative method was used to analyze Type I and III collagen mRNA in the left ventricular.
(1) Blood pressure: after the treatment, the systolic pressure and diastolic pressure were both increased in the group A and the group B (P < 0.05); while the two pressures were both lowered in the group C and the group D (P < 0.05), which was more obvious in the group C (P < 0.05). (2) According to HE and Masson staining, except for the group E, the myocardial hypertrophy and fibrosis could be found in the rest groups, in which the group C was the modest, followed by the group D, while the group A and the group B were more severe. (3) Concentration of ET-1: there were differences of concentration of ET-1 among 5 groups (P < 0.05), and the concentration value from high to low was the group A, B, C, D and E. (4) Type I collagen mRNA: the difference of level of Type I collagen mRNA between group C and D was not statistically significant (P > 0.05); compared with the group A and B, the level was lower in the group C; the level was the lowest in the group E. Type III collagen mRNA: the difference between the group A and B was not statistically significant (P > 0.05); compared with the group A, B and D, the level was lower in the group C.
The twirling-rotating reducing could reduce the systolic pressure and diastolic pressure in SHR, effectively prohibit the production of ET-1 and expression of Type I and III collagen mRNA, and it has more obvious inhibiting effect on Type III collagen mRNA. There is biological effect difference between twirling-rotating reinforcing and reducing technique.
探讨针刺捻转补泻手法对自发性高血压大鼠（SHR）心脏损害的效应差异。
将60只11周龄雄性SHR随机分为四组：模型对照组（A组）、捻转补法组（B组）、捻转泻法组（C组）和留针组（D组），每组15只。另取12只11周龄雄性Wistar大鼠作为空白对照组（E组）。A组和E组不针刺，仅给予抓取、束缚刺激，其余各组同样施加该刺激。B组大鼠采用捻转补法针刺“太冲”（LR 3）1分钟，然后留针9分钟；C组大鼠采用捻转泻法针刺“太冲”（LR 3）1分钟，然后留针9分钟；D组大鼠针刺“太冲”（LR 3）但不采用任何手法，然后留针10分钟。针刺前后每6天用血压计测量大鼠收缩压和舒张压。治疗28天后，采用HE和Masson染色观察左心室肥厚及心肌纤维化情况。采用ELISA法检测内皮素-1（ET-1）含量。采用PCR半定量法分析左心室Ⅰ型和Ⅲ型胶原mRNA。
（1）血压：治疗后，A组和B组收缩压和舒张压均升高（P < 0.05）；而C组和D组两项血压均降低（P < 0.05），C组更明显（P < 0.05）。（2）根据HE和Masson染色结果，除E组外，其余各组均可见心肌肥厚和纤维化，其中C组程度最轻，其次为D组，A组和B组较重。（3）ET-1浓度：5组间ET-1浓度存在差异（P < 0.05），浓度值从高到低依次为A组、B组、C组、D组、E组。（4）Ⅰ型胶原mRNA：C组和D组Ⅰ型胶原mRNA水平差异无统计学意义（P > 0.05）；与A组和B组相比，C组水平较低；E组水平最低。Ⅲ型胶原mRNA：A组和B组差异无统计学意义（P > 0.05）；与A组、B组和D组相比，C组水平较低。
捻转泻法可降低SHR的收缩压和舒张压，有效抑制ET-1的产生及Ⅰ型和Ⅲ型胶原mRNA的表达，对Ⅲ型胶原mRNA的抑制作用更明显。捻转补泻手法存在生物学效应差异。
