Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong 250012, P.R. China.
Mol Med Rep. 2020 May;21(5):2085-2094. doi: 10.3892/mmr.2020.11021. Epub 2020 Mar 11.
Periodontitis is a common inflammatory disorder affecting the tissues surrounding the teeth, which can lead to the destruction of periodontal tissue and tooth loss. Resveratrol, a natural phytoalexin, exerts multiple biological effects. For example, its anti‑inflammatory activity has been widely studied for the treatment of inflammatory bowel disease for a number of years. However, its effect on bone repair and new bone formation in an inflammatory microenvironment is not well understood. Accordingly, the effect of resveratrol on inflammation‑affected human periodontal ligament stem cells (hPDLSCs) requires further investigation. In the present study, the effect of tumor necrosis factor‑α (TNF‑α), resveratrol, or the combination of both on the osteogenic differentiation of hPDLSCs, as well as the underlying mechanisms involved, were investigated. Cell Counting Kit‑8 assay, alkaline phosphatase staining, Alizarin red staining, Oil Red O staining, reverse transcription‑quantitative PCR and western blotting were used in the present study. It was demonstrated that resveratrol enhanced hPDLSC osteogenesis and reversed the inhibitory effects of TNF‑α on this process. Further mechanistic studies indicated that resveratrol exerted anti‑inflammatory activity by activating the ERK1/2 pathway, decreasing the secretion of interleukin (IL)‑6 and IL‑8 induced by TNF‑α, and enhancing hPDLSCs osteogenesis. The present study suggested that resveratrol may be a novel and promising therapeutic choice for periodontitis.
牙周炎是一种常见的炎症性疾病，影响牙齿周围的组织，可导致牙周组织破坏和牙齿脱落。白藜芦醇是一种天然植物抗毒素，具有多种生物学作用。例如，其抗炎活性已被广泛研究多年，用于治疗炎症性肠病。然而，其在炎症微环境中对骨修复和新骨形成的影响尚不清楚。因此，白藜芦醇对受炎症影响的人牙周韧带干细胞（hPDLSCs）的影响需要进一步研究。在本研究中，研究了肿瘤坏死因子-α（TNF-α）、白藜芦醇或两者联合对 hPDLSCs 成骨分化的影响及其相关机制。本研究采用细胞计数试剂盒-8 检测法、碱性磷酸酶染色、茜素红染色、油红 O 染色、逆转录-定量 PCR 和 Western blot 法。结果表明，白藜芦醇增强了 hPDLSC 的成骨作用，并逆转了 TNF-α对该过程的抑制作用。进一步的机制研究表明，白藜芦醇通过激活 ERK1/2 通路发挥抗炎作用，减少 TNF-α诱导的白细胞介素（IL）-6 和 IL-8 的分泌，并增强 hPDLSCs 的成骨作用。本研究提示，白藜芦醇可能是治疗牙周炎的一种新的有前途的治疗选择。
