Costa Chiara, Briguglio Giusi, Giambò Federica, Catanoso Rosaria, Teodoro Michele, Caccamo Daniela, Fenga Concettina
Department of Clinical and Experimental Medicine, University of Messina, I‑98125 Messina, Italy.
Department of Biomedical and Dental Sciences and Morpho‑Functional Imaging, Occupational Medicine Section, University of Messina, I‑98125 Messina, Italy.
Int J Mol Med. 2020 Jun;45(6):1951-1959. doi: 10.3892/ijmm.2020.4541. Epub 2020 Mar 16.
Low levels of pesticides persist in the environment and can affect the health of exposed subjects. Oxidative stress is considered as one of the mechanisms responsible for the adverse effects on human health and some molecules may represent useful biomarkers for the evaluation of this physiological balance. This study investigated the role of these biomarkers, such as advanced oxidation protein products (AOPP), advanced glycation end‑products (AGE) and reactive oxygen metabolites (ROMs) in relation to genetic polymorphisms of paraoxonase (PON)1, PON2, glutathione S‑transferase pi 1 (GSTP1), glutathione S‑transferase theta 1 (GSTT1) and glutathione S‑transferase mu 1 (GSTM1). An increase in the levels of these biomarkers is usually inversely associated with the depletion of the biological antioxidant potential (BAP). The results revealed a statistically significant difference in the sex‑dependent variation of AGE, BAP, AOPP and ROM protein levels. Furthermore, an association between the PON2 S331C gene polymorphism and the serum levels of AOPP, ROMs and BAP was found. Thus, compared with AGE, the levels of AOPP and ROMs provided a more sensitive biomarker, with an improved association with the PON2 genotype. Such an association strengthen the importance of PON in the occurrence of oxidative stress. According to these results, an individual's genetic background may be taken into account for the health surveillance of individuals occupationally exposed to pesticides, in order to define a cluster of highly susceptible workers so as to guarantee greater protection.
低水平的农药持续存在于环境中，并可能影响接触者的健康。氧化应激被认为是对人类健康产生不利影响的机制之一，一些分子可能是评估这种生理平衡的有用生物标志物。本研究调查了这些生物标志物，如晚期氧化蛋白产物（AOPP）、晚期糖基化终产物（AGE）和活性氧代谢产物（ROMs）与对氧磷酶（PON）1、PON2、谷胱甘肽S-转移酶pi 1（GSTP1）、谷胱甘肽S-转移酶theta 1（GSTT1）和谷胱甘肽S-转移酶mu 1（GSTM1）基因多态性的关系。这些生物标志物水平的升高通常与生物抗氧化潜力（BAP）的消耗呈负相关。结果显示，AGE、BAP、AOPP和ROM蛋白水平在性别依赖性变化方面存在统计学上的显著差异。此外，还发现PON2 S331C基因多态性与血清AOPP、ROMs和BAP水平之间存在关联。因此，与AGE相比，AOPP和ROMs水平提供了更敏感的生物标志物，与PON2基因型的关联更强。这种关联强化了PON在氧化应激发生中的重要性。根据这些结果，在对职业接触农药的个体进行健康监测时，可考虑个体的遗传背景，以便确定一组高度易感工人，从而提供更好的保护。
