The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
Ziauddin University Faculty of Engineering, Science, Technology and Management (ZUFESTM), Department of Biomedical Engineering, Block B, North Nazimabad, Karachi, F-10374600, Pakistan.
Appl Biochem Biotechnol. 2024 Jun;196(6):3331-3343. doi: 10.1007/s12010-023-04649-w. Epub 2023 Aug 30.
Synthetic pesticides are employed to enhance agricultural production. Chronic exposure to organophosphate (OP) pesticides may be a source of health problems. The present study was designed to examine an association of GSTP1 (rs1695) polymorphism with OP pesticide chronic exposure. A case-control study was recruited with 250 subjects comprising exposed (n = 100) and controls (n = 150). A survey was conducted to determine the pesticide type to which workers had exposed. According to recorded survey assessment, two compounds of OP pesticides chloropyrifos and malathion were investigated in the blood samples of exposed study subjects using high-performance liquid chromatography (HPLC). For screening of genetic polymorphism in GSTP1 (rs1695) polymerase chain reaction, restriction length polymorphism (PCR-RFLP) and agarose gel electrophoresis were performed. Statistically, data were analyzed using SPSS v. 20.0 and MedCal© software. Total chrom navigator programmer was used for detection of OP residues in serum and local pesticide solution. chloropyrifos-OP pesticide residues were detected in serum of estimated chronically exposed subjects at 206 nm HPLC optimal conditions. The pattern of GSTP1 (rs1695) genotypic frequencies depicted that heterozygous genotype was higher in Chloropyrifos exposed subjects (0.56) when compared with controls (0.44). Statistical outcomes showed an insignificant association with GSTP1 (rs1695) polymorphism and chloropyrifos-OP pesticide toxicity (Fisher's exact test 1.0, p = 0.25). An insignificant allelic investigation reflected a protective effect of mutant allele G against chloropyrifos-OP pesticide toxicity in exposed subjects. Findings may be helpful in identifying bioaccumulated pesticide residues, but in studied Pakistani exposed workers, no significant association of GSTP1 (rs1695) variant with chloropyrifos-OPs was demonstrated.
合成农药被用于提高农业产量。长期接触有机磷（OP）农药可能是健康问题的一个来源。本研究旨在研究 GSTP1（rs1695）多态性与 OP 农药慢性暴露的相关性。采用病例对照研究，共招募 250 名受试者，包括暴露组（n=100）和对照组（n=150）。通过调查确定工人接触的农药类型。根据记录的调查评估，使用高效液相色谱法（HPLC）在暴露组研究对象的血液样本中检测到两种 OP 农药氯吡硫磷和马拉硫磷。为了筛选 GSTP1（rs1695）聚合酶链反应中的遗传多态性，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和琼脂糖凝胶电泳法。统计分析采用 SPSS v. 20.0 和 MedCal© 软件。使用总铬导航员程序检测血清和局部农药溶液中的 OP 残留。在估计的慢性暴露受试者的血清中，在 206nm HPLC 最佳条件下检测到氯吡硫磷-OP 农药残留。GSTP1（rs1695）基因型频率的模式表明，与对照组（0.44）相比，氯吡硫磷暴露组的杂合基因型更高（0.56）。统计结果显示，GSTP1（rs1695）多态性与氯吡硫磷-OP 农药毒性无显著相关性（Fisher 确切检验 1.0，p=0.25）。等位基因的显著性研究表明，突变型等位基因 G 对氯吡硫磷-OP 农药毒性有保护作用。研究结果可能有助于识别生物蓄积性农药残留，但在研究的巴基斯坦暴露工人中，未发现 GSTP1（rs1695）变异与氯吡硫磷-OPs 有显著相关性。
