Department of Oriental Medicine Biotechnology, College of Life Sciences, Graduate School of Biotechnology, Kyung Hee University, Gyeonggi, Korea.
Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Seoul, Korea.
J Med Food. 2020 May;23(5):491-498. doi: 10.1089/jmf.2019.4542. Epub 2020 Mar 18.
Changing consumption patterns and increasing health awareness, especially in Europe, are resulting in an increased demand for sesame seeds. In 2016, Asia imported the highest quantity of sesame seeds, followed by Europe and North America. We examined, for the first time, the effects of treatment with sesame oil and sesamin in hearing impairment models. Sesame oil exhibited an ameliorative effect on auditory impairment in a hair cell line in zebrafish and mice. In ototoxic zebrafish larvae, neuromasts and otic cells increased in numbers because of sesame oil. Furthermore, auditory function in noise-induced hearing loss (NIHL) was studied through auditory brainstem response to evaluate the therapeutic effects of sesame oil. Sesame oil reduced the hearing threshold shift in response to clicks and 8, 16-kHz tone bursts in NIHL mice. Auditory-protective effect of sesame oil was seen in zebrafish and mice; therefore, we used chromatographic analysis to study sesamin, which is the major effective factor in sesame oil. To investigate its effects related to auditory function, we studied the hearing-related gene, , using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) assay. Auditory cell proliferation was induced by treatment with sesame oil and sesamin using (Tectorin Alpha) regulation. The expression of increases in the apex area of the cochlear hair cells as they grow, and their activity is enhanced by sesame oil and sesamin. These results provide a novel mechanistic insight into the sesame oil activities and suggest that sesamin, the key constituent in sesame oil, is responsible for its auditory function related benefits, including protection of auditory cells and reversal of their impairments.
随着消费模式的改变和健康意识的提高，尤其是在欧洲，对芝麻的需求不断增加。2016 年，亚洲进口的芝麻数量最多，其次是欧洲和北美。我们首次研究了芝麻油和芝麻素在听力损伤模型中的作用。芝麻油对斑马鱼和小鼠的毛细胞系听力损伤具有改善作用。在耳毒性斑马鱼幼虫中，由于芝麻油的作用，神经嵴和耳石细胞数量增加。此外，通过听觉脑干反应研究噪声诱导听力损失（NIHL）中的听觉功能，以评估芝麻油的治疗效果。芝麻油可降低 NIHL 小鼠对 click 和 8、16-kHz 啁啾的听力阈值偏移。芝麻油烟对斑马鱼和小鼠均具有听觉保护作用；因此，我们使用色谱分析来研究芝麻素，这是芝麻油中的主要有效成分。为了研究其与听觉功能相关的作用，我们使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法研究了与听力相关的基因 。通过用芝麻油和芝麻素处理 （Tectorin Alpha）调节，诱导听觉细胞增殖。随着耳蜗毛细胞的生长， 的表达增加，芝麻油和芝麻素增强了其活性。这些结果为芝麻油的作用提供了新的机制见解，并表明芝麻油中的关键成分芝麻素负责其与听觉功能相关的益处，包括保护听觉细胞和逆转其损伤。
