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Sepsis impedes EAE disease development and diminishes autoantigen-specific naive CD4 T cells.脓毒症阻碍 EAE 疾病的发展，并减少自身抗原特异性幼稚 CD4 T 细胞。
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Rapid Light-Dependent Degradation of Fluorescent Dyes in Formulated Serum-Free Media.荧光染料在配方无血清培养基中的快速光依赖性降解
Immunohorizons. 2019 Dec 16;3(12):585-592. doi: 10.4049/immunohorizons.1900080.
2
Sepsis-Induced T Cell Immunoparalysis: The Ins and Outs of Impaired T Cell Immunity.脓毒症诱导的 T 细胞免疫麻痹：受损 T 细胞免疫的来龙去脉。
J Immunol. 2018 Mar 1;200(5):1543-1553. doi: 10.4049/jimmunol.1701618.
3
Cryopreservation of human whole blood allows immunophenotyping by flow cytometry up to 30days after cell isolation.人全血的冷冻保存可使细胞分离后长达30天通过流式细胞术进行免疫表型分析。
J Immunol Methods. 2018 Jan;452:32-38. doi: 10.1016/j.jim.2017.08.013. Epub 2017 Sep 18.
4
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.流式细胞术 APC 串联染料通过细胞依赖的机制降解。
Cytometry A. 2009 Oct;75(10):882-90. doi: 10.1002/cyto.a.20774.
5
Selecting fluorochrome conjugates for maximum sensitivity.选择荧光染料偶联物以实现最大灵敏度。
Cytometry A. 2004 Dec;62(2):169-73. doi: 10.1002/cyto.a.20092.
6
TNF/iNOS-producing dendritic cells mediate innate immune defense against bacterial infection.产生肿瘤坏死因子/诱导型一氧化氮合酶的树突状细胞介导针对细菌感染的固有免疫防御。
Immunity. 2003 Jul;19(1):59-70. doi: 10.1016/s1074-7613(03)00171-7.
7
Near-infrared dyes for six-color immunophenotyping by laser scanning cytometry.用于激光扫描细胞术六色免疫表型分析的近红外染料。
Cytometry. 2002 Jul 1;48(3):115-23. doi: 10.1002/cyto.10119.
8
Oxidative stress in malaria; implications for prevention and therapy.疟疾中的氧化应激；对预防和治疗的意义。
Pharm World Sci. 1996 Aug;18(4):121-9. doi: 10.1007/BF00717727.

Worry and FRET: ROS Production Leads to Fluorochrome Tandem Degradation and impairs Interpretation of Flow Cytometric Results.

作者信息

Jensen Isaac J, McGonagill Patrick W, Lefebvre Mitchell N, Griffith Thomas S, Harty John T, Badovinac Vladimir P

机构信息

Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, USA.

Department of Surgery, University of Iowa, Iowa City, IA, USA.

出版信息

Immunity. 2020 Mar 17;52(3):419-421. doi: 10.1016/j.immuni.2020.02.003.

DOI:10.1016/j.immuni.2020.02.003
PMID:32187510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7251922/
Abstract
摘要