Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Arkansas Children's Research Institute, Little Rock, AR 72202, USA.
Nutrients. 2020 Mar 16;12(3):781. doi: 10.3390/nu12030781.
Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families and (mostly ) and a decrease in in animals fed a methionine-restricted diet compared to controls. In females, family S24-7 was increased two-fold, while families , and were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome.
饮食蛋氨酸限制与改善动物模型的健康结果和延长寿命有关。我们之前已经表明，饮食中蛋氨酸的增加会导致肠道微生物组发生改变。肠道微生物组的组成是健康的决定因素，因此，我们假设饮食蛋氨酸限制也会引起小鼠微生物组的变化。在接受蛋氨酸限制饮食一个月后，五个月大的雄性和雌性 C57BL/6 小鼠的血清蛋氨酸水平降低，而体重没有变化。与对照组相比，我们发现喂食蛋氨酸限制饮食的动物的肝甲基化状态下降。这种下降与肝脏中 DNA 或蛋白质甲基化的变化无关。在雄性中，与对照组相比，喂食蛋氨酸限制饮食的动物中 和 （主要是 ）增加，而 减少。在雌性中，家族 S24-7 增加了两倍，而家族 、 和 则比对照组减少。总之，喂食蛋氨酸限制饮食一个月与肠道微生物组的显著性别特异性变化有关。
