Products Derived from Leaves Have In Vitro Antioxidant Activity and Protect Larvae against -Induced Injury.

The relevance of oxidative stress in the pathogenesis of several diseases (including inflammatory disorders) has traditionally led to the search for new sources of antioxidant compounds. In this work, we report the selection of fractions with high antioxidant action from (BT) leaf extracts. methods (DPPH and ABTS assays; determination of phenolic and flavonoid contents) were used to select products derived from with high antioxidant action. Then, the samples with the highest potentials were evaluated in a model of injury based on the inoculation of a lethal dose of heat-inactivated in larvae. Due to its higher antioxidant properties, the methanolic extract (BTME) was chosen to be fractionated using Sephadex LH-20 column-based chromatography. Two fractions from BTME (BTFC and BTFD) were the most active fractions. Pre-treatment with these fractions protected larvae of from the stress induced by inoculation of heat-inactivated . Similarly, BTFC and BTFD increased the lifespan of larvae infected with a lethal dose of enteroaggregative 042. NMR data indicated the presence of aliphatic compounds (terpenes, fatty acids, carbohydrates) and aromatic compounds (phenolic compounds). These findings suggested that products derived from leaves are promising candidates for the development of antioxidant and anti-infective agents able to treat oxidative-related dysfunctions.