Department of Infectious Diseases, Linyi People's Hospital, Linyi, 276034, Shandong, China.
Department of Internal Medicine, Linyi People's Hospital, Linyi, 276034, Shandong, China.
Eur J Med Res. 2020 Mar 18;25(1):9. doi: 10.1186/s40001-020-00408-3.
MicroRNAs (miRNAs) play important roles in the development and progression of sepsis. This study investigated the clinical value of miR-19b-3p in sepsis patients, and explored its role in regulating inflammatory responses in HUVECs cells.
103 patients with sepsis and 98 healthy individuals were recruited. qRT-PCR was used for the measurement of miR-19b-3p level. Cell viability was evaluated using CCK-8. The protein levels of TNF-α and IL-6 were measured using ELISA. Receiver operating characteristic (ROC) curve and logistic regression analysis were constructed to evaluate the diagnostic and prognostic values of miR-19b-3p in sepsis patients.
MiR-19b-3p level was significantly reduced in the serum from patients with sepsis compared with healthy controls (P < 0.001). Sepsis patients in the survival group had significantly high miR-19b-3p levels compared with the non-survival group (P < 0.001). MiR-19b-3p was of a good value in predicting sepsis risk, and was an independent prognostic factor for 28-day survival in sepsis patients (OR = 3.226, 95% CI 1.076-9.670, P = 0.037). MiR-19b-3p level was negatively associated with serum levels of IL-6 (r = - 0.852, P < 0.001) and TNF-α (r = - 0.761, P < 0.001). Overexpression of miR-19b-3p alleviated LPS-induced inflammatory response of HUVECs, which was reflected by the decrease of the levels of IL-6 and TNF-α induced by LPS treatment (P < 0.001).
MiR-19b-3p might be a potential biomarker for the early diagnosis and prognosis of sepsis patients. Overexpression of miR-19b-3p alleviated sepsis-induced inflammatory responses.
微小 RNA（miRNAs）在脓毒症的发生和发展中发挥着重要作用。本研究探讨了 miR-19b-3p 在脓毒症患者中的临床价值，并探讨了其在调节 HUVECs 细胞炎症反应中的作用。
招募了 103 例脓毒症患者和 98 例健康个体。使用 qRT-PCR 测量 miR-19b-3p 水平。使用 CCK-8 评估细胞活力。使用 ELISA 测量 TNF-α 和 IL-6 的蛋白水平。构建受试者工作特征（ROC）曲线和逻辑回归分析，以评估 miR-19b-3p 在脓毒症患者中的诊断和预后价值。
与健康对照组相比，脓毒症患者血清中的 miR-19b-3p 水平显著降低（P<0.001）。存活组的脓毒症患者的 miR-19b-3p 水平明显高于非存活组（P<0.001）。miR-19b-3p 具有很好的预测脓毒症风险的价值，并且是脓毒症患者 28 天生存率的独立预后因素（OR=3.226，95%CI 1.076-9.670，P=0.037）。miR-19b-3p 水平与血清中 IL-6（r=-0.852，P<0.001）和 TNF-α（r=-0.761，P<0.001）的水平呈负相关。过表达 miR-19b-3p 减轻了 LPS 诱导的 HUVECs 的炎症反应，这反映在 LPS 处理诱导的 IL-6 和 TNF-α水平的降低（P<0.001）。
miR-19b-3p 可能是脓毒症患者早期诊断和预后的潜在生物标志物。过表达 miR-19b-3p 减轻了脓毒症引起的炎症反应。
