Real Fernando, Capron Claude, Sennepin Alexis, Arrigucci Riccardo, Zhu Aiwei, Sannier Gérémy, Zheng Jonathan, Xu Lin, Massé Jean-Marc, Greffe Ségolène, Cazabat Michelle, Donoso Maribel, Delobel Pierre, Izopet Jacques, Eugenin Eliseo, Gennaro Maria Laura, Rouveix Elisabeth, Cramer Bordé Elisabeth, Bomsel Morgane
Mucosal Entry of HIV and Mucosal Immunity, Institut Cochin, Université de Paris, Paris, France.
INSERM U1016, Paris, France.
Sci Transl Med. 2020 Mar 18;12(535). doi: 10.1126/scitranslmed.aat6263.
In addition to hemostasis, human platelets have several immune functions and interact with infectious pathogens including HIV in vitro. Here, we report that platelets from HIV-infected individuals on combined antiretroviral drug therapy (ART) with low blood CD4 T cell counts (<350 cells/μl) contained replication-competent HIV despite viral suppression. In vitro, human platelets harboring HIV propagated the virus to macrophages, a process that could be prevented with the biologic abciximab, an anti-integrin αIIb/β3 Fab. Furthermore, in our cohort, 88% of HIV-infected individuals on ART with viral suppression and with platelets containing HIV were poor immunological responders with CD4 T cell counts remaining below <350 cells/μl for more than one year. Our study suggests that platelets may be transient carriers of HIV and may provide an alternative pathway for HIV dissemination in HIV-infected individuals on ART with viral suppression and poor CD4 T cell recovery.
除止血功能外，人类血小板还具有多种免疫功能，并在体外与包括HIV在内的感染性病原体相互作用。在此，我们报告，接受联合抗逆转录病毒药物治疗（ART）且血液CD4 T细胞计数较低（<350个细胞/μl）的HIV感染者的血小板，尽管病毒受到抑制，但仍含有具有复制能力的HIV。在体外，携带HIV的人类血小板将病毒传播给巨噬细胞，这一过程可用生物制剂阿昔单抗（一种抗整合素αIIb/β3 Fab）来预防。此外，在我们的队列中，88%接受ART且病毒受到抑制、血小板含有HIV的HIV感染者是免疫反应不佳者，其CD4 T细胞计数持续低于<350个细胞/μl超过一年。我们的研究表明，血小板可能是HIV的短暂携带者，并可能为接受ART且病毒受到抑制但CD4 T细胞恢复不佳的HIV感染者提供HIV传播的另一条途径。
