College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, China
State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science & Technology, Shijiazhuang, China.
mSphere. 2020 Mar 18;5(2):e00058-20. doi: 10.1128/mSphere.00058-20.
Substantial concentrations of penicillin V potassium (PVK) have been found in livestock manure, soil, and wastewater effluents, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, bacterial strains capable of degrading PVK were isolated from sludge and characterized. Strain X-2 was selected for biodegradation of PVK. Based on morphological observations and 16S rRNA gene sequencing, strain X-2 was identified as an strain. To enhance the PVK degradation ability of PVK, a whole-cell biodegradation process of X-2 was established and optimized. In the whole-cell biodegradation process, the optimal temperature and pH were 30°C and 7.0, respectively. Under the optimized conditions, the degradation rate using 0.5 mg/ml PVK reached 100% within 3 h. During biodegradation, two major metabolites were detected: penicilloic acid and phenolic acid. The present study provides a novel method for the biodegradation of PVK using strains, which represent promising candidates for the industrial biodegradation of PVK. Substantial concentrations of penicillin V potassium (PVK) have been found in the environment, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, antibiotic-degrading bacterial strains for PVK were isolated from sludge and characterized. was selected for the biodegradation of PVK with high efficiency. To enhance its PVK degradation ability, a whole-cell biodegradation process was established and optimized using The degradation rate with 0.5 mg/ml PVK reached 100% within 3 h. The potential biodegradation pathway was also investigated. To the best of our knowledge, the present study provides new insights into the biodegradation of PVK using an strain, a promising candidate strain for the industrial biodegradation of β-lactam antibiotics.
从污泥中分离和鉴定了具有降解青霉素 V 钾（PVK）能力的细菌菌株。选择 菌株 X-2 进行 PVK 的生物降解。根据形态观察和 16S rRNA 基因测序，菌株 X-2 被鉴定为 菌株。为了提高 PVK 的降解能力，建立并优化了 X-2 的全细胞生物降解工艺。在全细胞生物降解过程中，最佳温度和 pH 值分别为 30°C 和 7.0。在优化条件下，使用 0.5mg/ml PVK 时，3 h 内的降解率达到 100%。在生物降解过程中，检测到两种主要代谢物：青霉素酸和酚酸。本研究为利用 菌株进行 PVK 的生物降解提供了一种新方法，为 PVK 的工业生物降解提供了有前途的候选菌株。在环境中发现了大量的青霉素 V 钾（PVK），这可能对人类健康构成潜在威胁，并导致青霉素抗性细菌菌株的出现。在这项研究中，从污泥中分离和鉴定了用于 PVK 降解的抗生素降解细菌菌株。 菌株被选为具有高效降解 PVK 的菌株。为了提高其 PVK 降解能力，使用 建立并优化了全细胞生物降解工艺。使用 0.5mg/ml PVK 时，3 h 内的降解率达到 100%。还研究了潜在的生物降解途径。据我们所知，本研究为利用 菌株进行 PVK 生物降解提供了新的见解，这是一种用于β-内酰胺类抗生素工业生物降解的有前途的候选菌株。
