Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China.
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.
Int J Environ Res Public Health. 2019 Jun 19;16(12):2166. doi: 10.3390/ijerph16122166.
(1) Background: Antibiotics are frequently used on farm animals, making animal husbandry a relatively large source of antibiotic pollution of the environment. The present study aims to isolate and acclimatize antibiotic-degrading bacterial strains for penicillin V potassium (PVK) from the contaminated soil of a pig farm. (2) Methods: Bacterial strains were isolated and acclimatized by continuous enrichment of cultures with PVK as the sole carbon source. The antibiotic susceptibility test, thiol mercury salt ultraviolet spectrophotometry (TMSUS), morphological observations, and 16S rDNA sequence analysis were used to identify and characterize the isolated strains. (3) Results: Four bacterial isolates (denoted as LM-1, LM-2, LM-3, LM-4) were obtained, and two of them (LM-1, LM-2) with the highest degradation rates were identified to belong to the same genera as . These two isolates were found to be resistant to PVK antibiotic in an antibiotic sensitivity test. The TMSUS indicated that the strains LM-1 and LM-2 had good performance in PVK degradation (68% for LM-1, 66% for LM-2 in 48 h) when the initial PVK concentration was about 100 μg/mL. (4) Conclusions: Two bacterial strains isolated from the soil on a pig farm are effective in degrading PVK and can be potentially used for bioremediation of PVK antibiotic-contaminated soils.
（1）背景：抗生素在农场动物中经常使用，使畜牧业成为环境中抗生素污染的相对较大来源。本研究旨在从养猪场污染土壤中分离和驯化能够降解青霉素 V 钾（PVK）的抗生素降解细菌菌株。（2）方法：通过连续富集培养物，以 PVK 作为唯一碳源来分离和驯化细菌菌株。抗生素敏感性测试、硫汞盐紫外分光光度法（TMSUS）、形态观察和 16S rDNA 序列分析用于鉴定和表征分离出的菌株。（3）结果：获得了四个细菌分离株（分别表示为 LM-1、LM-2、LM-3、LM-4），其中两个（LM-1、LM-2）具有最高的降解率，被鉴定为属于相同的属。这些两种分离株在抗生素敏感性测试中对 PVK 抗生素具有抗性。TMSUS 表明，当初始 PVK 浓度约为 100μg/mL 时，菌株 LM-1 和 LM-2 在 PVK 降解方面表现出色（LM-1 为 68%，LM-2 为 66%，48 小时）。（4）结论：从养猪场土壤中分离出的两种细菌菌株能够有效降解 PVK，可潜在用于 PVK 抗生素污染土壤的生物修复。
