Department of Medicine, University of California Los Angeles, Los Angeles, California, USA.
CytoAnalytics, Denver, Colorado, USA.
J Infect Dis. 2020 Jul 23;222(4):690-694. doi: 10.1093/infdis/jiaa127.
To investigate the role of serum cytokine assays to distinguish between active from treated syphilis among serofast patients, we recruited individuals into a prospective cohort study. Participants underwent routine syphilis screening. We selected specimens from a majority cohort of serofast participants with treated and active syphilis. We analyzed specimens with a 62-cytokine multiplex bead-based enzyme-linked immunosorbent assay. Cytokines, brain-derived neurotrophic factor and tumor necrosis factor β, were most predictive. We built a decision tree that was 82.4% accurate, 100% (95% confidence interval, 82%-100%) sensitive, and 45% (18%-75%) specific. Our decision tree differentiated between serum specimens from serofast participants with treated syphilis versus active syphilis.
为了研究血清细胞因子检测在血清固定患者中区分活动性和治疗性梅毒的作用，我们招募了一些个体进入前瞻性队列研究。参与者接受了常规梅毒筛查。我们从大多数治疗性和活动性梅毒血清固定患者中选择了标本。我们用 62 种细胞因子的多重微珠酶联免疫吸附试验分析了标本。细胞因子、脑源性神经营养因子和肿瘤坏死因子β最具预测性。我们建立了一个决策树，其准确率为 82.4%，敏感度为 100%（95%置信区间，82%-100%），特异性为 45%（18%-75%）。我们的决策树区分了血清固定患者中治疗性梅毒和活动性梅毒血清标本。
