Noorolahi Zohre, Sahari Mohammad Ali, Barzegar Mohsen, Ahmadi Gavlighi Hassan
Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
J Food Biochem. 2020 Jun;44(6):e13208. doi: 10.1111/jfbc.13208. Epub 2020 Mar 18.
Polyphenolic compounds (present in different parts of the plant) have beneficial properties such as antioxidant and inhibition of key enzymes. In this research, antioxidant and anti-lipase activity of pistachio green hull (PGH) extract was investigated. Fractionation of PGH on Sephadex LH-20 furnished a tannin enriched fraction with higher antioxidant activity respect to that of the extract and of the non-tannin fraction. UHPL/MS analyses showed the presence of phenolic compounds including galloyl-O-hexoside, galloyl-shikimic acid, galloylquinic acid, and gallic acid in tannin fraction. PGH-extract was an un-competitive inhibitor against porcine pancreatic lipase so that its IC value was 2.26 mg/ml. In the same phenol amount (490 µg), anti-lipase activity of the tannin fraction was also more than non-tannin fraction and crude PGH-extract. This is probably due to the presence of some active polyphenolic compounds such as gallic acid. PRACTICAL APPLICATIONS: Pistachio is native to the arid regions of Central and West Asia including Iran. The green hull is main by-product of pistachio industry that has numerous phenolic compounds. Our results showed that the pistachio green hull extract has antioxidant and anti-lipase activity and these activities in its tannin fraction were higher than non-tannin fraction. Therefore, the PGH extract and its tannin fraction can be used as potential substitutes of anti-obesity drugs. This allows the use of pistachio processing waste and reduces the amount of waste.
多酚类化合物（存在于植物的不同部位）具有抗氧化和抑制关键酶等有益特性。在本研究中，对开心果绿壳（PGH）提取物的抗氧化和抗脂肪酶活性进行了研究。PGH在葡聚糖凝胶LH - 20上的分级分离得到了一个富含单宁的级分，其抗氧化活性高于提取物和非单宁级分。超高效液相色谱/质谱分析表明，单宁级分中存在酚类化合物，包括没食子酰 - O - 己糖苷、没食子酰莽草酸、没食子酰奎宁酸和没食子酸。PGH提取物是猪胰脂肪酶的非竞争性抑制剂，其IC值为2.26 mg/ml。在相同酚含量（490 μg）下，单宁级分的抗脂肪酶活性也高于非单宁级分和粗PGH提取物。这可能是由于存在一些活性多酚类化合物，如没食子酸。实际应用：开心果原产于包括伊朗在内的中亚和西亚干旱地区。绿壳是开心果产业的主要副产品，含有大量酚类化合物。我们的数据表明，开心果绿壳提取物具有抗氧化和抗脂肪酶活性，且其单宁级分中的这些活性高于非单宁级分。因此，PGH提取物及其单宁级分可作为抗肥胖药物的潜在替代品。这使得开心果加工废料得以利用，减少了废料量。
