Foundation Prof. Antonio Imbesi, University of Messina, Piazza Pugliatti 1, 98122, Messina, Italy.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Giovanni Palatucci, 98168, Messina, Italy.
Plant Foods Hum Nutr. 2021 Mar;76(1):111-117. doi: 10.1007/s11130-021-00883-x. Epub 2021 Feb 26.
In the present study an acidified methanol pistachio hull extract was investigated for antioxidant and inhibitory effects on melanin biosynthesis by in vitro and in vivo assays. The chromatographic analysis revealed that cyanidin-3-O-galactoside represents the main compound (98.37%). The pistachio hull extract efficiently inhibits the mono and diphenolase activity of mushroom tyrosinase (IC= 141.07 and 116.08 μg/mL, respectively) and it was able, thanks to its strong antioxidant and free-radical scavenging activities, to hinder the L-DOPA auto-oxidation in a concentration-dependent manner (125-500 μg/mL). Results of in vivo assay showed that the treatment with pistachio hull extract (10 μg/mL) reduced pigmentation in zebrafish embryos at early stages of development (60.01% of inhibition vs control). In conclusion, these findings suggest that the ripe pistachio hull may be considered as a promising source of antioxidant and skin whitening agents for the development of new products useful in preventing the pigmentation disorders in humans and/or to improve the food quality.
在本研究中，通过体外和体内试验研究了酸化甲醇巴旦木壳提取物的抗氧化和抑制黑色素生物合成的作用。色谱分析表明，矢车菊素-3-O-半乳糖苷是主要化合物（98.37%）。巴旦木壳提取物能有效抑制蘑菇酪氨酸酶的单酚酶和二酚酶活性（IC=141.07 和 116.08 μg/mL），并能通过其强大的抗氧化和自由基清除活性，以浓度依赖的方式阻碍 L-DOPA 的自动氧化（125-500 μg/mL）。体内试验结果表明，巴旦木壳提取物（10 μg/mL）处理能减少斑马鱼胚胎发育早期的色素沉着（与对照组相比抑制率为 60.01%）。综上所述，这些发现表明成熟的巴旦木壳可能被认为是一种有前途的抗氧化剂和皮肤美白剂的来源，可用于开发新产品，以预防人类色素沉着紊乱和/或改善食品质量。
