Kan Yuxuan, Zeng Mengnan, Zhang Beibei, Li Benke, Wang Shenchao, Wang Yangyang, Xu Ruiqi, Wu Yuanyuan, Zheng Xiaoke, Feng Weisheng
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases, Henan & Education Ministry of China, Zhengzhou 450046, China.
Evid Based Complement Alternat Med. 2020 Feb 27;2020:6020817. doi: 10.1155/2020/6020817. eCollection 2020.
B. L. Burtt is a traditional Chinese medicine. Previous studies in our laboratory showed that alleviated symptoms of Alzheimer's disease (AD) in a rat model of AD and increased healthy rats' urine volume. The aims of this study were to explore the diuretic activity of different extracts from and to identify the underlying mechanisms of action. Different doses of a extract (CF-L, CF-M, and CF-H) were administered orally to male KM mice in a single dose. In another procedure, (CF), water extract, and 20%, 30%, and 40% ethanol extracts of (CF-WE, CF-20, CF-30, and CF-40) were administered orally daily for 5 days. The urinary excretion rate, osmolality, and electrolyte levels in urine and serum, renal expression of aquaporins (AQPs), apoptosis-related protein, and MAPK-related protein were analyzed. The results showed that single doses of CF-M and CF-H increased urinary volume significantly, as well as daily administration of CF, CF-WE, CF-20, CF-30, and CF-40. Furthermore, CF-20 and CF-30 increased the concentration of Na in the urine. Treatment with CF-40 increased the urine osmolality and Na and Cl concentrations and decreased the concentration of Na in the serum. Also, CF, CF-WE, CF-20, CF-30, and CF-40 decreased the renal expression of AQPs, as well as the ratios of Bcl-2/Bax, p-ERK/ERK, p-JNK/JNK, and p-p38/p38. In sum, the medium and high doses of the extract and CF-WE, CF-20, CF-30, and CF-40 were found to have a diuretic activity. They may inhibit the renal expression of AQPs and apoptosis-related proteins by inhibiting the MAPK signaling pathway, thereby achieving diuretic effects.
B.L. Burtt是一种中药。我们实验室之前的研究表明，其可缓解阿尔茨海默病（AD）大鼠模型的症状，并增加健康大鼠的尿量。本研究的目的是探索B.L. Burtt不同提取物的利尿活性，并确定其潜在作用机制。将不同剂量的B.L. Burtt提取物（CF-L、CF-M和CF-H）以单剂量口服给予雄性KM小鼠。在另一个实验中，将B.L. Burtt（CF）、水提取物以及B.L. Burtt的20%、30%和40%乙醇提取物（CF-WE、CF-20、CF-30和CF-40）每日口服给药5天。分析尿排泄率、尿渗透压、尿和血清中的电解质水平、肾水通道蛋白（AQPs）的表达、凋亡相关蛋白和丝裂原活化蛋白激酶（MAPK）相关蛋白。结果表明，单剂量的CF-M和CF-H以及每日给予CF、CF-WE、CF-20、CF-30和CF-40均显著增加尿量。此外，CF-20和CF-30增加尿中Na的浓度。CF-40处理增加尿渗透压以及Na和Cl的浓度，并降低血清中Na的浓度。此外，CF、CF-WE、CF-20、CF-30和CF-40降低肾AQPs的表达以及Bcl-2/Bax、p-ERK/ERK、p-JNK/JNK和p-p38/p38的比值。总之，发现B.L. Burtt提取物的中高剂量以及CF-WE、CF-20、CF-30和CF-40具有利尿活性。它们可能通过抑制MAPK信号通路来抑制肾AQPs和凋亡相关蛋白的表达，从而实现利尿作用。
