Abd-Elrahim Batran Samah
Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Toxicol Rep. 2020 Mar 2;7:453-459. doi: 10.1016/j.toxrep.2020.03.002. eCollection 2020.
Environmental ozone (O) exposure has adverse effects on different body systems. This experimental work aimed to study the effect(s) of O exposure on the olfactory bulbs (OB) of Swiss Webster and C57BL/6J mouse strains, using Western blot technique. Both mice strains were exposed to different O doses for different number of exposures and durations. The results indicated that O exposure caused a significant increase in the level of the proteins involved in the oxidative stress state such as 4-hydroxynonenal (4HNE) and Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), in addition to the total OB proteins in Swiss Webster mouse strain. However, this effect was not observed in C57BL/6J mouse strain. Furthermore, CYP1A1 was completely absent in the Green fluorescent protein (GFP) C57BL/6J O exposed mice. Moreover, O exposure caused a significant decrease in the body weight of the tested mice from the two strains.
环境臭氧（O）暴露会对不同身体系统产生不良影响。本实验旨在利用蛋白质印迹技术研究臭氧暴露对瑞士韦伯斯特小鼠品系和C57BL/6J小鼠品系嗅球（OB）的影响。两种小鼠品系均暴露于不同剂量的臭氧中，暴露次数和持续时间各不相同。结果表明，臭氧暴露导致瑞士韦伯斯特小鼠品系中参与氧化应激状态的蛋白质水平显著升高，如4-羟基壬烯醛（4HNE）和细胞色素P450 1A1亚家族A多肽1（CYP1A1），此外嗅球总蛋白水平也升高。然而，在C57BL/6J小鼠品系中未观察到这种效应。此外，在暴露于臭氧的绿色荧光蛋白（GFP）C57BL/6J小鼠中，CYP1A1完全缺失。此外，臭氧暴露导致两个品系受试小鼠的体重显著下降。
