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生成 Fsp1（成纤维细胞特异性蛋白 1）-Flpo 转基因小鼠品系。

Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain.

机构信息

INSERM U1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France.

CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France.

出版信息

Genesis. 2020 May;58(5):e23359. doi: 10.1002/dvg.23359. Epub 2020 Mar 19.

DOI:10.1002/dvg.23359
PMID:32191380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7317532/
Abstract

Recombination systems represent a major breakthrough in the field of genetic model engineering. The Flp recombinases (Flp, Flpe, and Flpo) bind and cleave DNA Frt sites. We created a transgenic mouse strain ([Fsp1-Flpo]) expressing the Flpo recombinase in fibroblasts. This strain was obtained by random insertion inside mouse zygotes after pronuclear injection. Flpo expression was placed under the control of the promoter of Fsp1 (fibroblast-specific protein 1) gene, whose expression starts after gastrulation at Day 8.5 in cells of mesenchymal origin. We verified the correct expression and function of the Flpo enzyme by several ex vivo and in vivo approaches. The [Fsp1-Flpo] strain represents a genuine tool to further target the recombination of transgenes with Frt sites specifically in cells of mesenchymal origin or with a fibroblastic phenotype.

摘要

重组系统代表了遗传模型工程领域的重大突破。Flp 重组酶（Flp、Flpe 和 Flpo）结合并切割 DNA Frt 位点。我们创建了一种表达 Flpo 重组酶的转基因小鼠品系 ([Fsp1-Flpo])，该品系通过原核注射后随机插入到受精卵中获得。Flpo 表达受 Fsp1（成纤维细胞特异性蛋白 1）基因启动子的控制，该基因在胚胎发生第 8.5 天开始在中胚层来源的细胞中表达。我们通过多种离体和体内方法验证了 Flpo 酶的正确表达和功能。[Fsp1-Flpo]品系代表了一种真正的工具，可以进一步将 Frt 位点的转基因重组专门靶向中胚层来源的细胞或具有成纤维细胞表型的细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/5f80de4787e7/DVG-58-e23359-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/1ee787e471e2/DVG-58-e23359-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/adcaf995ad11/DVG-58-e23359-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/aab97db07128/DVG-58-e23359-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/5f80de4787e7/DVG-58-e23359-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/1ee787e471e2/DVG-58-e23359-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/adcaf995ad11/DVG-58-e23359-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/aab97db07128/DVG-58-e23359-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/548f/7317532/5f80de4787e7/DVG-58-e23359-g004.jpg

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2
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Genesis. 2018 Aug;56(8):e23243. doi: 10.1002/dvg.23243. Epub 2018 Aug 23.
3
Loss of from neurons regulating hypoxic and hypercapnic chemoresponses causes neonatal respiratory failure in mice.
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Elife. 2018 Jul 4;7:e38455. doi: 10.7554/eLife.38455.
4
Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele.利用Pdx1-Flp重组酶敲入等位基因构建胰腺癌模型。
PLoS One. 2017 Sep 21;12(9):e0184984. doi: 10.1371/journal.pone.0184984. eCollection 2017.
5
Identification of markers for quiescent pancreatic stellate cells in the normal human pancreas.正常人类胰腺中静止胰腺星状细胞标志物的鉴定。
Histochem Cell Biol. 2017 Oct;148(4):359-380. doi: 10.1007/s00418-017-1581-5. Epub 2017 May 25.
6
Identification of Serotonergic Neuronal Modules that Affect Aggressive Behavior.影响攻击行为的5-羟色胺能神经元模块的鉴定。
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7
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Neuron. 2016 Sep 21;91(6):1228-1243. doi: 10.1016/j.neuron.2016.08.021. Epub 2016 Sep 8.
8
Cre Recombinase and Other Tyrosine Recombinases.Cre 重组酶和其他酪氨酸重组酶。
Chem Rev. 2016 Oct 26;116(20):12785-12820. doi: 10.1021/acs.chemrev.6b00077. Epub 2016 May 10.
9
Bhlhb5::flpo allele uncovers a requirement for Bhlhb5 for the development of the dorsal cochlear nucleus.Bhlhb5::flpo等位基因揭示了背侧耳蜗核发育对Bhlhb5的需求。
Dev Biol. 2016 Jun 15;414(2):149-60. doi: 10.1016/j.ydbio.2016.04.028. Epub 2016 May 3.
10
Expanding the power of recombinase-based labeling to uncover cellular diversity.扩展基于重组酶标记的能力以揭示细胞多样性。
Development. 2015 Dec 15;142(24):4385-93. doi: 10.1242/dev.129981. Epub 2015 Nov 19.