Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Student Research Committee, Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Biomed Mater Res A. 2020 Aug 1;108(8):1662-1672. doi: 10.1002/jbm.a.36932. Epub 2020 Apr 3.
Dynamic-based systems are bio-designed in order to mimic the micro-environments of the bone tissue. There is limited direct comparison between perfusion and perfusion-rotation forces in designing a bioreactor. Hence, in current study, we aimed to compare given bioreactors for bone regeneration. Two types of bioreactors including rotating & perfusion and perfusion bioreactors were designed. Mesenchymal stem cells derived from buccal fat pad were loaded on a gelatin/β-Tricalcium phosphate scaffold. Cell-scaffold constructs were subjected to different treatment condition and place in either of the bioreactors. Effect of different dynamic conditions on cellular behavior including cell proliferation, cell adhesion, and osteogenic differentiation were assessed. Osteogenic assessment of scaffolds after 24 days revealed that rotating & perfusion bioreactor led to significantly higher expression of OCN and RUNX2 genes and also greater amount of ALP and collagen I protein production compared to static groups and perfusion bioreactor. Observation of cellular sheets which filled the scaffold porosities in SEM images, approved the better cell responses to rotating & perfusion forces of the bioreactor. The outcomes demonstrated that rotating & perfusion bioreactor action on bone regeneration is much preferable than perfusion bioreactor. Therefore, it seems that exertion of multi-stimuli is more effective for bone engineering.
为了模拟骨组织的微环境，动态系统是经过生物设计的。在设计生物反应器时，灌注和灌注-旋转力之间没有直接的比较。因此，在本研究中，我们旨在比较用于骨再生的给定生物反应器。设计了两种类型的生物反应器，包括旋转和灌注以及灌注生物反应器。从颊脂垫中分离的间充质干细胞被加载到明胶/β-磷酸三钙支架上。细胞-支架构建体被置于不同的处理条件下，并放置在生物反应器中的任何一个中。评估不同动态条件对细胞行为的影响，包括细胞增殖、细胞黏附和成骨分化。在第 24 天对支架进行成骨评估后发现，与静态组和灌注生物反应器相比，旋转和灌注生物反应器导致 OCN 和 RUNX2 基因的表达显著增加，同时 ALP 和胶原 I 蛋白的产生量也增加。SEM 图像中观察到细胞片填充在支架孔隙中，证明了生物反应器旋转和灌注力对细胞的更好反应。结果表明，旋转和灌注生物反应器对骨再生的作用优于灌注生物反应器。因此，施加多刺激似乎对骨工程更有效。
