Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; and College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
Anal Chem. 2020 Apr 21;92(8):5795-5802. doi: 10.1021/acs.analchem.9b05299. Epub 2020 Mar 31.
Nonspecific adsorption is of great concern for electrochemical biosensors performing in complex biological media, and various antifouling materials have been introduced into the sensing interfaces to improve the antifouling capability of different biosensors. However, for most of the biosensors with antifouling materials and sensing probes coexisting in the sensing interfaces, either the antifouling materials will impair the sensing performances or the sensing probes will affect the antifouling ability. Herein, a facile and efficient antifouling biosensor was developed based on a newly designed three-in-one peptide with anchoring, antifouling, and recognizing capabilities. One end of the designed peptide is a unique anchoring part that is rich in amine groups, and this part can be anchored to the poly(3,4-ethylenedioxythiophene) (PEDOT)-citrate film electrodeposited on a glassy carbon electrode. The other end of the peptide is a recognizing part that can specifically bind to the aminopeptidase N (APN) and human hepatocellular carcinoma cells (HepG2 cells). Meanwhile, the middle part of the peptide, together with the anchoring part, was designed to be antifouling. With this designed multifunctional peptide, highly sensitive and low-fouling biosensors capable of assaying target APN and HepG2 cells in complex biological media can be easily prepared, with detection limits of 0.4 ng·mL and 20 cells·mL, respectively. This antifouling biosensor is feasible for practical target detection in real complex samples, and it is highly expected that this peptide designing strategy may be extended to the development of various antifouling biosensors.
非特异性吸附是电化学生物传感器在复杂生物介质中应用时需要重点关注的问题，因此人们引入了各种抗污材料来改善不同生物传感器的抗污能力。然而，对于大多数具有抗污材料和传感探针共存于传感界面的生物传感器来说，抗污材料要么会损害传感性能，要么传感探针会影响抗污能力。在此，基于一种新设计的具有锚固、抗污和识别功能的三合一肽，开发了一种简单高效的抗污生物传感器。设计的肽的一端是一个独特的锚固部分，富含氨基，该部分可以锚定在聚（3,4-乙二氧基噻吩）（PEDOT）-柠檬酸修饰的玻碳电极上。肽的另一端是识别部分，可特异性结合氨肽酶 N（APN）和人肝癌细胞（HepG2 细胞）。同时，肽的中间部分与锚固部分一起被设计为抗污部分。利用这种设计的多功能肽，可以轻松制备出能够在复杂生物介质中检测目标 APN 和 HepG2 细胞的高灵敏、低污染生物传感器，检测限分别为 0.4ng·mL 和 20 个细胞·mL。这种抗污生物传感器可用于实际复杂样品中的实际目标检测，预计这种肽设计策略可能会扩展到各种抗污生物传感器的开发。
