Chen Min, Song Zhen, Han Rui, Li Yang, Luo Xiliang
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Biosens Bioelectron. 2021 Apr 15;178:113016. doi: 10.1016/j.bios.2021.113016. Epub 2021 Jan 21.
The nonspecific adsorption and accumulation of biomolecules on electrode interfaces remains a challenge for sensitive and accurate detection of disease markers in complex biological media, and it is highly desired to develop antifouling biosensors capable of assaying targets in complicated real liquids. Herein, an efficient and simple antifouling biosensor was constructed based on a self-designed Y-shaped peptide. The Y-shaped peptide was designed with two branches: one branch with the peptide sequence of EKEKEKE for antifouling, and the other branch with the peptide sequence of HWRGWVA for recognizing of human IgG. Under optimized experimental conditions, electrodes modified with Y-shaped peptides exhibited excellent antifouling and electrochemical sensing performances. The developed biosensor was able to effectively resist biofouling in various protein solutions and even serum samples, and the linear range of the biosensor for human IgG detection was from 100 pg mL to 10 μg mL, with a relatively low limit of detection of 32 pg mL (S/N = 3). The antifouling biosensor possesses the capability of assaying human IgG in real serum samples, and this strategy of developing low fouling biosensors based on Y-shaped peptides can be readily expanded to the construction of other biosensing systems for different targets.
生物分子在电极界面上的非特异性吸附和积累仍然是在复杂生物介质中灵敏且准确地检测疾病标志物的一项挑战，因此迫切需要开发能够在复杂实际液体中检测目标物的抗污染生物传感器。在此，基于自行设计的Y形肽构建了一种高效且简便的抗污染生物传感器。该Y形肽设计有两个分支：一个分支具有用于抗污染的EKEKEKE肽序列，另一个分支具有用于识别人类IgG的HWRGWVA肽序列。在优化的实验条件下，用Y形肽修饰的电极表现出优异的抗污染和电化学传感性能。所开发的生物传感器能够有效抵抗各种蛋白质溶液甚至血清样品中的生物污染，该生物传感器检测人类IgG的线性范围为100 pg/mL至10 μg/mL，检测限相对较低，为32 pg/mL（S/N = 3）。该抗污染生物传感器具有检测实际血清样品中人类IgG的能力，并且这种基于Y形肽开发低污染生物传感器的策略可以很容易地扩展到构建用于不同目标物的其他生物传感系统。
