Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States.
Org Lett. 2020 Apr 3;22(7):2626-2629. doi: 10.1021/acs.orglett.0c00597. Epub 2020 Mar 19.
This work describes the development of a highly stable and pH-responsive probe for lysine modification. The scaffold has marked stability in the presence of several biological nucleophiles and across a wide pH range (2-12). Several functional analogs showed robust labeling of a protein at pH > 9. Taken together, our system displays versatility and can be easily adapted for variety of applications, while demonstrating stability suitable for a wide range of biologically compatible systems.
本工作描述了一种高度稳定且对 pH 响应的赖氨酸修饰探针的开发。该支架在存在几种生物亲核试剂和宽 pH 范围（2-12）的情况下具有显著的稳定性。几种功能类似物在 pH > 9 时显示出对蛋白质的强标记。总之，我们的系统显示出多功能性，可以轻松适应各种应用，同时展示出适合广泛生物相容体系的稳定性。
