Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea.
J Med Food. 2020 Mar;23(3):266-272. doi: 10.1089/jmf.2019.4654.
Mulberry ( L.) fruits have long been used in traditional medicine and as edible berries in many countries. This study investigated the antiadipogenic effect of high hydrostatic pressure mulberry fruit extract (MFE) during 3T3-L1 adipocyte differentiation. MFE decreased lipid and triglyceride accumulation and glycerol-3-phosphate dehydrogenase activity. The mRNA expression levels of genes related to adipogenesis, such as the adipocyte protein 2, proliferator-activated receptor-, and CCAAT/enhancer binding protein-, were suppressed by MFE. They also reduced microRNA (miR)-21 and miR-143 expression, which are involved in adipogenesis. In contrast, adenosine monophosphate-activated protein kinase (AMPK) activity was increased by MFE. These results suggested that MFE may suppress adipogenesis through modulating miR-21/143 expression and AMPK activity in 3T3-L1 adipocytes, which may be useful as antiobesity food agents.
桑（Mulberry）果在传统医学中被广泛应用，并且在许多国家被当作可食用的浆果。本研究调查了高静压桑果提取物（MFE）在 3T3-L1 脂肪细胞分化过程中的抗脂肪生成作用。MFE 可减少脂肪和甘油三酯的积累以及甘油-3-磷酸脱氢酶的活性。MFE 还抑制与脂肪生成相关的基因，如脂肪细胞蛋白 2、增殖激活受体-和 CCAAT/增强子结合蛋白-的 mRNA 表达。MFE 还降低了参与脂肪生成的 microRNA（miR）-21 和 miR-143 的表达。相反，MFE 增加了单磷酸腺苷激活的蛋白激酶（AMPK）的活性。这些结果表明，MFE 可能通过调节 3T3-L1 脂肪细胞中的 miR-21/143 表达和 AMPK 活性来抑制脂肪生成，这可能有助于开发作为抗肥胖食品的成分。
