Combined Amoebicidal Effect of Atorvastatin and Commercial Eye Drops against Neff: In Vitro Assay Based on Mixture Design.

The establishment of an effective therapeutic agent against keratitis (AK), remains until present, an issue to be solved due to the existence of a cyst stage in the life cycle of . Moreover, the effectiveness of the current standard therapeutic agents varies depending on the tested strains and its resistance pattern. In the present study, two 10-point augmented simplex-centroid designs were used to formulate a three-component mixture system using water, atorvastatin, and Diclofenaco-lepori or Optiben. The amoebicidal effects and -induced toxicity in a eukaryotic cell line were determined for all experiments. The optimal mixture to inhibit the parasite without inducing toxicity was established in the first plan as 30% Optiben, 63.5% atorvastatin, and 3.1% water. As for the second experimental design, the optimal mixture to inhibit with lower toxicity effect was composed of 17.6% Diclofenaco-lepori and 82.4% atorvastatin.