Sifaoui Ines, Capote Yanes Eulalia Capote, Reyes-Batlle María, Rodríguez-Expósito Rubén L, Piñero José E, Lorenzo-Morales Jacob
Instituto Universitario De Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, La Laguna, Tenerife, 38203 Islas Canarias, Spain.
Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, 38203 Islas Canarias, Spain.
Pathogens. 2020 Mar 17;9(3):219. doi: 10.3390/pathogens9030219.
The establishment of an effective therapeutic agent against keratitis (AK), remains until present, an issue to be solved due to the existence of a cyst stage in the life cycle of . Moreover, the effectiveness of the current standard therapeutic agents varies depending on the tested strains and its resistance pattern. In the present study, two 10-point augmented simplex-centroid designs were used to formulate a three-component mixture system using water, atorvastatin, and Diclofenaco-lepori or Optiben. The amoebicidal effects and -induced toxicity in a eukaryotic cell line were determined for all experiments. The optimal mixture to inhibit the parasite without inducing toxicity was established in the first plan as 30% Optiben, 63.5% atorvastatin, and 3.1% water. As for the second experimental design, the optimal mixture to inhibit with lower toxicity effect was composed of 17.6% Diclofenaco-lepori and 82.4% atorvastatin.
由于在其生命周期中存在囊肿阶段，目前针对角膜炎（AK）的有效治疗药物的研发仍然是一个有待解决的问题。此外，当前标准治疗药物的有效性因测试菌株及其耐药模式而异。在本研究中，使用了两个10点增强单纯形重心设计，以水、阿托伐他汀和双氯芬酸-兔或Optiben配制了一种三组分混合系统。在所有实验中测定了对真核细胞系的杀阿米巴作用和诱导的毒性。在第一个方案中确定的抑制寄生虫而不诱导毒性的最佳混合物为30%Optiben、63.5%阿托伐他汀和3.1%水。至于第二个实验设计，具有较低毒性作用的抑制最佳混合物由17.6%双氯芬酸-兔和82.4%阿托伐他汀组成。
