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从Pall. ex Link提取物中发现其对[具体物种1]和[具体物种2]具有杀阿米巴活性，且对人角膜细胞毒性低。

Pall. ex Link Extract Reveals Amoebicidal Activity against and with Low Toxicity to Human Corneal Cells.

作者信息

Lê Hương Giang, Hwang Buyng Su, Choi Ji-Su, Jeong Yong Tae, Kang Jung-Mi, Võ Tuấn Cường, Oh Young Taek, Na Byoung-Kuk

机构信息

Department of Parasitology and Tropical Medicine, and Institute of Health Science, Gyeongsang National University College of Medicine, Jinju 52727, Republic of Korea.

Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Republic of Korea.

出版信息

Microorganisms. 2024 Aug 13;12(8):1658. doi: 10.3390/microorganisms12081658.

DOI:10.3390/microorganisms12081658
PMID:39203500
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11356916/
Abstract

keratitis (AK) is a sight-threatening and difficult-to-treat ocular infection. The significant side effects of current AK treatments highlight the urgent need to develop a safe and effective AK medication. In this study, the amoebicidal activity of Pall. ex Link extract (ISE) against was examined and its specific amoebicidal mechanism was explored. ISE induced significant morphological changes in trophozoites and exhibited amoebicidal activity against and . ISE was further fractionated into five subfractions by sequential extraction with -hexane, chloroform, ethyl acetate, -butanol, and water, and their amoebicidal activities and underlying amoebicidal mechanisms were investigated. The -butanol subfraction of ISE (ISE-BuOH) displayed selective amoebicidal activity against the species with minimal cytotoxicity in human corneal cells (HCE-2). ISE-BuOH triggered apoptosis-like programmed cell death (PCD) in amoebae, characterized by DNA fragmentation, increased ROS production, and caspase-3 activity elevation. ISE-BuOH also demonstrated a partial cysticidal effect against the amoeba species. ISE-BuOH could be a promising candidate in the development of therapeutic drugs for AK.

摘要

棘阿米巴角膜炎（AK）是一种威胁视力且难以治疗的眼部感染。当前AK治疗方法的显著副作用凸显了开发安全有效的AK药物的迫切需求。在本研究中，检测了苍白杆菌提取物（ISE）对棘阿米巴的杀阿米巴活性，并探索了其具体的杀阿米巴机制。ISE诱导了棘阿米巴滋养体显著的形态变化，并对棘阿米巴和耐格里属阿米巴表现出杀阿米巴活性。通过依次用正己烷、氯仿、乙酸乙酯、正丁醇和水萃取，ISE进一步被分离为五个亚组分，并研究了它们的杀阿米巴活性及其潜在的杀阿米巴机制。ISE的正丁醇亚组分（ISE-BuOH）对棘阿米巴属物种表现出选择性杀阿米巴活性，对人角膜细胞（HCE-2）的细胞毒性最小。ISE-BuOH在阿米巴中引发了类似凋亡的程序性细胞死亡（PCD），其特征为DNA片段化、活性氧生成增加和半胱天冬酶-3活性升高。ISE-BuOH对阿米巴物种也表现出部分杀包囊作用。ISE-BuOH可能是开发AK治疗药物的一个有前景的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/69ce7adfaedf/microorganisms-12-01658-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/d636c141232f/microorganisms-12-01658-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/e5222b990550/microorganisms-12-01658-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/dafe27486c10/microorganisms-12-01658-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/8341dbca249c/microorganisms-12-01658-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/2ef1b4750bd1/microorganisms-12-01658-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/cf85f178b838/microorganisms-12-01658-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/6fd97dc52bc8/microorganisms-12-01658-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/64485b3ab560/microorganisms-12-01658-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/69ce7adfaedf/microorganisms-12-01658-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/d636c141232f/microorganisms-12-01658-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/e5222b990550/microorganisms-12-01658-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/dafe27486c10/microorganisms-12-01658-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/8341dbca249c/microorganisms-12-01658-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/2ef1b4750bd1/microorganisms-12-01658-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/cf85f178b838/microorganisms-12-01658-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/6fd97dc52bc8/microorganisms-12-01658-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/64485b3ab560/microorganisms-12-01658-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab98/11356916/69ce7adfaedf/microorganisms-12-01658-g009.jpg

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