Mitsuwan Watcharapong, Sin Chea, Keo Samell, Sangkanu Suthinee, de Lourdes Pereira Maria, Jimoh Tajudeen O, Salibay Cristina C, Nawaz Muhammad, Norouzi Roghayeh, Siyadatpanah Abolghasem, Wiart Christophe, Wilairatana Polrat, Mutombo Polydor Ngoy, Nissapatorn Veeranoot
School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand.
Akkhraratchakumari Veterinary College and Research Center of Excellence in Innovation of Essential Oil, Walailak University, Nakhon Si Thammarat, Thailand.
Heliyon. 2021 May 10;7(5):e06976. doi: 10.1016/j.heliyon.2021.e06976. eCollection 2021 May.
Plants with medicinal properties have been used in the treatment of several infectious diseases, including infections. The medicinal properties of Cambodian plant extracts; and were investigated against . A total of 39 plant extracts were evaluated and, as a result, 22 extracts showed positive anti- activity. Of the 22 extracts, 9 and 4 extracts showed anti- activity against trophozoites and cysts of , respectively. The minimum inhibitory concentration of and extracts against trophozoites and cysts was 500 and 1,000 μg/mL, respectively. The combination of at 1/4×MIC with chlorhexidine at 1/8×MIC demonstrated a synergistic effect against trophozoites, but partial synergy against cysts. A 40% reduction in trophozoites and 60% of cysts adhered to the plastic surface treated with both extracts at 1/2×MIC were noted comparing to the control (P < 0.05). Furthermore, a reduction of 80% and 90% of trophozoites adhered to the surface was observed after pre-treatment with and extracts, respectively. A 90% of cysts adhered to the surface was decreased with pre-treatment of at 1/2×MIC (P < 0.05). A 75% of trophozoites and cysts from adhered to the surface were removed after treatment with both extracts at 4×MIC (P < 0.05). In the model of contact lens, 1 log cells/mL of trophozoites and cysts was significantly decreased post-treatment with both extracts compared to the control. Trophozoites showed strong loss of acanthopodia and thorn-like projection pseudopodia, while cysts demonstrated retraction and folded appearance treated with both extracts when observed by SEM, which suggests the potential benefits of the medicinal plants and as an option treatment against infections.
具有药用特性的植物已被用于治疗多种传染病，包括感染。对柬埔寨植物提取物的药用特性进行了针对[某种病原体]的研究。共评估了39种植物提取物，结果有22种提取物显示出阳性抗[病原体]活性。在这22种提取物中，分别有9种和4种提取物对[病原体]的滋养体和包囊显示出抗[病原体]活性。[两种植物提取物名称]提取物对滋养体和包囊的最低抑菌浓度分别为500和1000μg/mL。[一种植物提取物名称]在1/4×MIC与洗必泰在1/8×MIC的组合对滋养体表现出协同作用，但对包囊表现出部分协同作用。与对照相比，在1/2×MIC下用两种提取物处理的塑料表面上，滋养体减少了40%，包囊减少了60%（P<0.05）。此外，在用[一种植物提取物名称]和[另一种植物提取物名称]提取物预处理后，观察到附着在表面的滋养体分别减少了80%和90%。在1/2×MIC下用[一种植物提取物名称]预处理后，附着在表面的包囊减少了90%（P<0.05）。在用4×MIC的两种提取物处理后，来自[病原体名称]的75%的滋养体和包囊从表面被去除（P<0.05）。在隐形眼镜模型中，与对照相比，用两种提取物处理后，滋养体和包囊的1 log细胞/mL显著减少。当通过扫描电子显微镜观察时，滋养体显示出棘状伪足和刺状突起伪足的强烈丧失，而包囊在用两种提取物处理后表现出收缩和折叠外观，这表明药用植物[两种植物名称]作为治疗[病原体]感染的一种选择具有潜在益处。
