Zuppolini Simona, Cruz-Maya Iriczalli, Guarino Vincenzo, Borriello Anna
Institute for Polymers, Composites and Biomaterials (IPCB)-National Research Council of Italy, V.le Kennedy 54, 80125 Naples, Italy.
J Funct Biomater. 2020 Mar 17;11(1):19. doi: 10.3390/jfb11010019.
In recent years, mussel adhesive proteins have attracted much attention because they can form strong adhesive interface interactions with various substrates in a wet environment. Inspired by their catechol- and amine-based molecular structure, polydopamine (PDA), a dopamine derived synthetic eumelanin polymer, was recognized as a suitable bio-interface coating. PDA was successfully used to improve adhesion due to the availability of copious functional groups for covalently immobilizing biomolecules and anchoring reactive species and ions. Recently, it has been demonstrated that PDA and its derivatives can be successfully used for the surface modification of implants interfaces to modulate in vitro cellular responses in order to enhance the in vivo functionality of biomedical implants (i.e., prosthesis). Herein, we propose the development of multifunctional scaffolds based on polyε-caprolactone (PCL) electrospun fibers coated with PDA via electro fluid dynamic methods, by optimizing polymerization/oxidation reactions capable of driving PDA self-assembly, and, ultimately, investigating the effects on cell response. Morphological analyses have confirmed the possibility to obtain different surface topographies as a function of the coating process while in vitro studies proved the ability of PDA coating to interact with cells no compromising in vitro viability. In perspective, in vitro conductive properties of fibers will be further investigated in order to validate their promising use as bioconductive interfaces for tissue engineering applications.
近年来，贻贝粘附蛋白备受关注，因为它们能够在潮湿环境中与各种底物形成强大的粘附界面相互作用。受其基于儿茶酚和胺的分子结构启发，聚多巴胺（PDA），一种由多巴胺衍生的合成真黑素聚合物，被认为是一种合适的生物界面涂层。由于有大量官能团可用于共价固定生物分子以及锚定反应性物种和离子，PDA已成功用于改善粘附性。最近，已证明PDA及其衍生物可成功用于植入物界面的表面改性，以调节体外细胞反应，从而增强生物医学植入物（即假体）的体内功能。在此，我们提出通过优化能够驱动PDA自组装的聚合/氧化反应，基于通过电流体动力学方法涂覆有PDA的聚ε-己内酯（PCL）电纺纤维开发多功能支架，并最终研究其对细胞反应的影响。形态学分析证实了根据涂层工艺获得不同表面形貌的可能性，而体外研究证明了PDA涂层与细胞相互作用而不损害体外活力的能力。展望未来，将进一步研究纤维的体外导电性能，以验证它们作为组织工程应用的生物导电界面的潜在用途。
