Peinemann Jan Christoph, Rhee Chaeyoung, Shin Seung Gu, Pleissner Daniel
Sustainable Chemistry (Resource Efficiency), Institute of Sustainable and Environmental Chemistry, Leuphana University of Lüneburg, Universitätsallee 1, C13.203, 21335 Lüneburg, Germany.
Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongnam National University of Science and Technology, 6 Naedong-ro 139beon-gil, Naedong-myeon, Jinju, South Korea.
Bioresour Technol. 2020 Jun;306:123175. doi: 10.1016/j.biortech.2020.123175. Epub 2020 Mar 10.
This work presents examples of non-sterile mixed culture fermentation of food waste with a cultivated indigenous consortium (IC) gained from food waste, which produces lactic and acetic acids, combined with Saccharomyces cerevisiae, which produces ethanol. All results are flanked by microbial analysis to monitor changes in microbial community. At pH 6 and inoculated with yeast or IC, or both mixed sugars conversion was equal to 71%, 51%, or 67%, respectively. Under pH unregulated conditions metabolic yields were 71%, 67%, or up to 81%. While final titer of acetic acid was not affected by pH (100-200 mM), ethanol and lactic acid titers were. Using mixed culture and pH 6, sugars were almost equally used for formation of ethanol and lactic acid (400-500 mM). However, under pH unregulated conditions 80% of the substrate was converted into ethanol (900-1000 mM).
这项工作展示了利用从食物垃圾中获得的驯化本土菌群（IC）对食物垃圾进行非无菌混合培养发酵的实例，该菌群可产生乳酸和乙酸，再结合酿酒酵母，后者可产生乙醇。所有结果均辅以微生物分析，以监测微生物群落的变化。在pH值为6且接种酵母或IC或两者的情况下，混合糖转化率分别为71%、51%或67%。在pH值未调节的条件下，代谢产率为71%、67%或高达81%。虽然乙酸的最终滴度不受pH值影响（100 - 200 mM），但乙醇和乳酸滴度受其影响。使用混合培养且pH值为6时，糖类几乎同等程度地用于乙醇和乳酸的形成（400 - 500 mM）。然而，在pH值未调节的条件下，80%的底物转化为乙醇（900 - 1000 mM）。
