State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China; Key Laboratory of Plant Nutrition and the Agri-Environment in Northwest China, Ministry of Agriculture, Yangling, Shaanxi Province, 712100, PR China.
Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, PR China.
J Hazard Mater. 2020 Jul 5;393:122489. doi: 10.1016/j.jhazmat.2020.122489. Epub 2020 Mar 7.
Soil microbial community structures and enzymatic activity are important indicators for judging biochemical process intensity and soil quality. Non-thermal discharge plasma (NDP), an advanced oxidation technique, has received great attention in soil remediation. Potential impact of NDP treatment on microbial community structures and enzymatic activities in uncontaminated soil samples was evaluated in this study. The NDP treatment significantly altered soil microbial community structures and enzymatic activity. The exposure of soil samples to NDP decreased the enzymatic activities including glutamic acid enzyme, arylsulphatase, leucine enzyme, acetyl glucosaccharase, glucosaccharase, cellulose, phosphatase, and ligninase. The contents of some soil microbes including Gram-negative bacteria, Fungi, Anaerobes, AM Fungi, Eukaryotes, and Actinomycetes also decreased after NDP treatment, as well as the diversity index and equitability index of the soil microbes. In addition, the total phospholipid fatty acid (PLFA), Fungi PLFA, bacteria PLFA, and Actinomycetes PLFA of the soil microbes were also reduced after treatment. Principal component analysis confirmed these changes. Actinomycetes and Fungi were the most sensitive microbes to the NDP, and Phosphatase activity and Cellulose activity were relatively insensitive to the NDP. The potential impacts could be attributed to chemically active substances and ultraviolet irradiation.
土壤微生物群落结构和酶活性是判断生化过程强度和土壤质量的重要指标。非热放电等离子体（NDP）作为一种先进的氧化技术，在土壤修复中受到了广泛关注。本研究评估了 NDP 处理对无污染土壤样品中微生物群落结构和酶活性的潜在影响。NDP 处理显著改变了土壤微生物群落结构和酶活性。土壤样品暴露于 NDP 会降低谷氨酸酶、芳基硫酸酯酶、亮氨酸酶、乙酰葡萄糖苷酶、葡萄糖苷酶、纤维素、磷酸酶和木质素酶等酶的活性。NDP 处理后，一些土壤微生物的含量也有所下降，如革兰氏阴性菌、真菌、厌氧菌、丛枝菌根真菌、真核生物和放线菌，土壤微生物的多样性指数和均度指数也有所下降。此外，土壤微生物的总磷脂脂肪酸（PLFA）、真菌 PLFA、细菌 PLFA 和放线菌 PLFA 也在处理后减少。主成分分析证实了这些变化。放线菌和真菌是对 NDP 最敏感的微生物，而磷酸酶活性和纤维素活性对 NDP 相对不敏感。这种潜在的影响可能归因于化学活性物质和紫外线辐射。
