Laboratory of Entomology, Fiocruz Rondônia, Brazil and PGBIOEXP/PNPD, Federal University Foundation of Rondônia, Porto Velho, Brazil.
Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China.
Sci Rep. 2020 Mar 19;10(1):4994. doi: 10.1038/s41598-020-61733-5.
Anopheles gambiae and Aedes aegypti are perhaps the best studied mosquito species and important carriers of human malaria and arbovirus, respectively. Mosquitoes have daily rhythms in behaviors and show a wide range of activity patterns. Although Anopheles is known to be principally nocturnal and Aedes principally diurnal, details of mosquito activity are not easily assayed in the laboratory. We recently described FlyBox, a simple tracking system for assaying Drosophila locomotor activity rhythms and thought that it might also be applicable to monitoring mosquito activity. Indeed, we show here that FlyBox can easily, conveniently, affordably and accurately measure the activity of Anopheles as well as Aedes over several days. The resulting profiles under light-dark as well as constant darkness conditions are compatible with results in the literature, indicating that this or similar systems will be useful in the future for more detailed studies on a range of insect species and under more diverse laboratory conditions.
冈比亚按蚊和埃及伊蚊可能是研究得最好的蚊子物种，分别是人类疟疾和虫媒病毒的重要载体。蚊子的行为具有每日节律，并表现出广泛的活动模式。尽管众所周知，按蚊主要是夜间活动，而埃及伊蚊主要是白天活动，但在实验室中，蚊子的活动细节不容易检测到。我们最近描述了 FlyBox，这是一种用于检测果蝇运动活动节律的简单跟踪系统，我们认为它也可能适用于监测蚊子的活动。事实上，我们在这里展示了 FlyBox 可以轻松、方便、经济高效且准确地测量几天内的按蚊和埃及伊蚊的活动。在光暗和持续黑暗条件下得到的图谱与文献中的结果相吻合，这表明这种或类似的系统将在未来对一系列昆虫物种在更多不同的实验室条件下进行更详细的研究中非常有用。
