Friedrich Schiller University Jena, Institute of Microbiology, 07745 Jena, Germany; Microverse Cluster, Friedrich Schiller University Jena, 07743 Jena, Germany.
Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-4417, USA.
Cell Chem Biol. 2024 Sep 19;31(9):1571-1577. doi: 10.1016/j.chembiol.2024.07.002. Epub 2024 Aug 1.
The study of small, regulatory RNAs (sRNA) that act by base-pairing with target RNAs in bacteria has been steadily advancing, particularly with the availability of more and more transcriptome and RNA-RNA interactome datasets. While the characterization of multiple sRNAs has helped to elucidate their mechanisms of action, these studies also are providing insights into protein function, control of metabolic flux, and connections between metabolic pathways as we will discuss here. In describing several examples of the metabolic insights gained, we will summarize the different types of base-pairing sRNAs including mRNA-derived sRNAs, sponge RNAs, RNA mimics, and dual-function RNAs as well as suggest how information about sRNAs could be exploited in the future.
对细菌中通过与靶 RNA 碱基配对起作用的小调控 RNA（sRNA）的研究一直在稳步推进，特别是随着越来越多的转录组和 RNA-RNA 相互作用组数据集的出现。虽然对多个 sRNA 的特征描述有助于阐明其作用机制，但这些研究也为我们将在这里讨论的蛋白质功能、代谢通量控制以及代谢途径之间的联系提供了新的见解。在描述获得的一些代谢见解的例子时，我们将总结不同类型的碱基配对 sRNA，包括 mRNA 衍生的 sRNA、海绵 RNA、RNA 模拟物和双功能 RNA，并提出未来如何利用 sRNA 的信息。
