Department of Bariatric and Metabolic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Diabetes Res Clin Pract. 2020 Apr;162:108116. doi: 10.1016/j.diabres.2020.108116. Epub 2020 Mar 16.
Alterations in gut microbiota have been associated with improvements in blood glucose due to bariatric surgery.
Our aim is to identify specific gut microbiota that contribute to the remission of T2D after RYGB and SG.
Rats fed a high-fat diet (HFD) were administered a low dose of streptozotocin as T2D models; next, their caecum content was collected 8 weeks after RYGB and SG. We also used case-control of gut microbial profiles of T2D patients and healthy people by collecting the mucosal-luminal interface from the ascending colon. Samples were profiled by 16S rRNA gene sequencing.
RYGB and SG reduced weight and improved glucose and insulin tolerance. Principal coordinate analysis showed that SG and, especially, RYGB cause changes in the composition of gut microbiota. We found that family Alcaligenaceae (genus Sutterella) was significantly decreased in the ascending colon of patients with T2D and increased after RYGB surgery in the caecum of T2D rats as shown by a linear discriminant analysis effect size (LEfSe) analysis, with no significant changes after SG. This result might benefit the improvement of glycometabolism.
RYGB can significantly reduce weight, improve glycometabolism and change the composition of the gut microbiota. Sutterella may have beneficial effects on glycometabolism in T2D patients after RYGB.
肠道微生物群的改变与减重手术后血糖改善有关。
我们旨在确定特定的肠道微生物群，这些微生物群有助于 RYGB 和 SG 后 2 型糖尿病的缓解。
高脂饮食（HFD）喂养的大鼠给予低剂量链脲佐菌素作为 2 型糖尿病模型；然后，在 RYGB 和 SG 后 8 周收集盲肠内容物。我们还通过从升结肠收集黏膜-腔界面，使用 2 型糖尿病患者和健康人的肠道微生物谱的病例对照来研究。通过 16S rRNA 基因测序对样本进行分析。
RYGB 和 SG 减轻体重，改善葡萄糖和胰岛素耐量。主坐标分析显示，SG，特别是 RYGB，导致肠道微生物群组成发生变化。我们发现，2 型糖尿病患者升结肠中的 Alcaligenaceae 科（Sutterella 属）显著减少，而在 2 型糖尿病大鼠的盲肠中 RYGB 手术后增加，通过线性判别分析效应大小（LEfSe）分析表明，SG 后没有显著变化。这一结果可能有益于改善糖代谢。
RYGB 可显著减轻体重、改善糖代谢并改变肠道微生物群的组成。Sutterella 可能对 RYGB 后 2 型糖尿病患者的糖代谢有益。
