Department of General Surgery, Shanghai Fengxian Central Hospital, Affiliated to Southern Medical University, Shanghai, China.
Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Surg Obes Relat Dis. 2018 May;14(5):584-593. doi: 10.1016/j.soard.2018.01.012. Epub 2018 Jan 17.
Changes in gut microbiota induced by bariatric surgery have been associated with metabolic benefits.
Our aim was to identify specific gut microbiota that may contribute to the improvement of type 2 diabetes (T2D) after Roux-en-Y gastric bypass (RYGB).
Laboratories of Shanghai Diabetes Institute and Shanghai Sixth People's Hospital.
Diabetic rats induced via a high-fat diet and low-dose streptozotocin administration were randomized to RYGB or sham surgery, and stool samples were collected at baseline and at postoperative week 8. The fecal microbiota was profiled using 16S ribosomal RNA gene sequencing. Additionally, we performed a case-control study of the gut microbial community profiles of T2D patients compared with those of healthy individuals via 16S ribosomal RNA gene sequencing of mucosal-luminal interface samples collected from the ascending colon during colonoscopy.
RYGB significantly reduced the weight and improved glucose tolerance and insulin sensitivity in diabetic rats. Principal coordinate analysis showed that RYGB caused marked alterations in the gut microbiota. The RYGB group was postoperatively enriched for Bacteroidetes, Proteobacteria, Fusobacteria, and Actinobacteria, whereas the sham surgery group was enriched for Firmicutes and Verrucomicrobia. Based on the gut microbial patterns in the T2D patients, we found that the family Coriobacteriaceae within Actinobacteria might contribute to the beneficial effects of RYGB on T2D.
RYGB significantly improves glucose metabolism and alters the gut microbiota. Moreover, the family Coriobacteriaceae may partly mediate the beneficial effects of RYGB on T2D and thus possibly contribute to the development of novel bacteria-based therapeutic approaches.
减重手术引起的肠道微生物群变化与代谢益处有关。
我们旨在确定可能有助于胃旁路手术后 2 型糖尿病（T2D）改善的特定肠道微生物群。
上海糖尿病研究所和上海第六人民医院的实验室。
通过高脂肪饮食和低剂量链脲佐菌素给药诱导糖尿病大鼠，并随机分为胃旁路手术（RYGB）或假手术组，基线和术后第 8 周采集粪便样本。使用 16S 核糖体 RNA 基因测序分析粪便微生物群。此外，我们通过结肠镜检查时从升结肠收集的黏膜腔界面样本的 16S 核糖体 RNA 基因测序，对 T2D 患者和健康个体的肠道微生物群落图谱进行了病例对照研究。
RYGB 显著减轻了糖尿病大鼠的体重，改善了葡萄糖耐量和胰岛素敏感性。主坐标分析表明，RYGB 导致肠道微生物群发生明显改变。RYGB 组术后富含拟杆菌门、变形菌门、梭杆菌门和放线菌门，而假手术组富含厚壁菌门和疣微菌门。基于 T2D 患者的肠道微生物模式，我们发现放线菌门内的科里氏菌科可能有助于 RYGB 对 T2D 的有益作用。
RYGB 显著改善葡萄糖代谢并改变肠道微生物群。此外，科里氏菌科可能部分介导 RYGB 对 T2D 的有益作用，从而可能有助于开发新的基于细菌的治疗方法。
