Plant Breeding Institute, Kiel University, Am Botanischen Garten 1-9, Kiel 24118, Germany.
CeBiTec &Faculty of Biology, Bielefeld University, Universitätsstraße 25, Bielefeld 33615, Germany.
Nat Commun. 2017 Jun 6;8:15708. doi: 10.1038/ncomms15708.
Rapid identification of agronomically important genes is of pivotal interest for crop breeding. One source of such genes are crop wild relative (CWR) populations. Here we used a CWR population of <200 wild beets (B. vulgaris ssp. maritima), sampled in their natural habitat, to identify the sugar beet (Beta vulgaris ssp. vulgaris) resistance gene Rz2 with a modified version of mapping-by-sequencing (MBS). For that, we generated a draft genome sequence of the wild beet. Our results show the importance of preserving CWR in situ and demonstrate the great potential of CWR for rapid discovery of causal genes relevant for crop improvement. The candidate gene for Rz2 was identified by MBS and subsequently corroborated via RNA interference (RNAi). Rz2 encodes a CC-NB-LRR protein. Access to the DNA sequence of Rz2 opens the path to improvement of resistance towards rhizomania not only by marker-assisted breeding but also by genome editing.
快速鉴定农艺重要基因对作物育种至关重要。这些基因的一个来源是作物野生近缘种（CWR）群体。在这里，我们利用在自然栖息地中采集的近 200 株野生甜菜（B. vulgaris ssp. maritima）的 CWR 群体，使用改良的图谱测序（MBS）方法来鉴定糖甜菜（Beta vulgaris ssp. vulgaris）的抗性基因 Rz2。为此，我们生成了野生甜菜的草图基因组序列。我们的结果表明，原地保护 CWR 的重要性，并证明了 CWR 对于快速发现与作物改良相关的因果基因具有巨大潜力。通过 MBS 鉴定候选基因 Rz2，随后通过 RNA 干扰（RNAi）进行验证。Rz2 编码一个 CC-NB-LRR 蛋白。获得 Rz2 的 DNA 序列为不仅通过标记辅助选择，还通过基因组编辑来提高抗根肿病的能力开辟了道路。
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