Xu Xiongfeng, Qiu Bo, Yi Peng, Li Huajie
Department of Orthopedic Surgery, The Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Oncol Lett. 2020 Mar;19(3):1751-1758. doi: 10.3892/ol.2020.11270. Epub 2020 Jan 8.
Osteosarcoma (OS) is a primary malignant bone tumor that predominantly occurs in adolescents. Different types of OS tumor are highly malignant, associated with a poor prognosis and are invasive with blood-vessel dissemination characteristics, thus affected patients are prone to early lung metastasis. MicroRNAs (miRNAs/miR) are small non-coding RNA molecules that act as oncogenes or tumor suppressors during tumor development. The present study investigated the role of miR-206 in OS development. Bioinformatics analysis demonstrated that miR-206 was upregulated in OS and thus may serve as a risk factor for cancer prognosis. Subsequently, in response to miR-206 overexpression, differentially expressed genes were screened and analyzed using the Database for Annotation, Visualization and Integrated Discovery, Gene Ontology enrichment analysis, the Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction network construction, in order to identify key miR-206 targets. The results demonstrated that high miR-206 expression inhibited OS cell proliferation, which was associated with a good patient prognosis. Thus, miR-206 may serve as a potential target for OS treatment, in order to improve early disease diagnosis.
骨肉瘤（OS）是一种主要发生于青少年的原发性恶性骨肿瘤。不同类型的骨肉瘤肿瘤恶性程度高，预后差，具有血管侵袭性和转移特性，因此患者易早期发生肺转移。微小RNA（miRNAs/miR）是一类小的非编码RNA分子，在肿瘤发生发展过程中发挥癌基因或抑癌基因的作用。本研究探讨了miR-206在骨肉瘤发生发展中的作用。生物信息学分析表明，miR-206在骨肉瘤中表达上调，可能是癌症预后的危险因素。随后，针对miR-206过表达，利用注释、可视化与整合发现数据库、基因本体富集分析、京都基因与基因组百科全书通路及蛋白质-蛋白质相互作用网络构建，筛选并分析差异表达基因，以鉴定关键的miR-206靶点。结果表明，miR-206高表达抑制骨肉瘤细胞增殖，这与患者良好预后相关。因此，miR-206可能成为骨肉瘤治疗的潜在靶点，以改善疾病早期诊断。
