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微小RNA-500a在结直肠癌中的致癌作用。

The oncogenic role of microRNA-500a in colorectal cancer.

作者信息

Li Yang, Chen Zhong

机构信息

First Department of General Surgery, Chengdu Shangjin Nan Fu Hospital, Chengdu, Sichuan 510117, P.R. China.

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

出版信息

Oncol Lett. 2020 Mar;19(3):1799-1805. doi: 10.3892/ol.2020.11291. Epub 2020 Jan 10.

DOI:10.3892/ol.2020.11291
PMID:32194673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7039136/
Abstract

Colorectal cancer (CRC) is a common and lethal disease, and microRNAs (miRNAs/miRs) serve an important role in the pathogenesis of CRC. miR-500a is a novel miRNA, and although its function has been studied in hepatocellular carcinoma, the function of miR-500a in CRC remains unknown. In the present study, the function of miR-500a in CRC was investigated. The expression levels of miR-500a in cells and tissues were investigated using reverse transcription-quantitative PCR. Cell proliferation was tested using MTT assay and migration was assessed using Transwell systems. The results revealed that there were higher levels of miR-500a in tumor tissue compared with in normal tissue. Inhibition of miR-500a suppressed cell growth and migration, whereas overexpression of miR-500a promoted cell growth and migration. Additionally, it was revealed that miR-500a may target the 3'-untranslated region of the phosphatase and tensin homolog gene. In conclusion, the present study demonstrated that miR-500a may serve an oncogenic role in CRC.

摘要

结直肠癌（CRC）是一种常见的致命疾病，微小RNA（miRNAs/miRs）在CRC的发病机制中发挥重要作用。miR-500a是一种新型微小RNA，尽管其在肝细胞癌中的功能已得到研究，但miR-500a在CRC中的功能仍不清楚。在本研究中，对miR-500a在CRC中的功能进行了研究。使用逆转录定量PCR检测细胞和组织中miR-500a的表达水平。使用MTT法检测细胞增殖，并使用Transwell系统评估细胞迁移。结果显示，与正常组织相比，肿瘤组织中miR-500a的水平更高。抑制miR-500a可抑制细胞生长和迁移，而miR-500a的过表达则促进细胞生长和迁移。此外，研究表明miR-500a可能靶向磷酸酶和张力蛋白同源基因的3'-非翻译区。总之，本研究表明miR-500a可能在CRC中发挥致癌作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/6520119b13f4/ol-19-03-1799-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/63ac0ddac456/ol-19-03-1799-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/3746767eb401/ol-19-03-1799-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/c3e9579473aa/ol-19-03-1799-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/6520119b13f4/ol-19-03-1799-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/63ac0ddac456/ol-19-03-1799-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/3746767eb401/ol-19-03-1799-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/c3e9579473aa/ol-19-03-1799-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/7039136/6520119b13f4/ol-19-03-1799-g03.jpg

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本文引用的文献

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World J Gastroenterol. 2018 Jul 21;24(27):2949-2973. doi: 10.3748/wjg.v24.i27.2949.
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MicroRNA-1296 Facilitates Proliferation, Migration And Invasion Of Colorectal Cancer Cells By Targeting SFPQ.微小RNA-1296通过靶向SFPQ促进结肠癌细胞的增殖、迁移和侵袭。
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MicroRNA-500a Promotes the Progression of Hepatocellular Carcinoma by Post-Transcriptionally Targeting BID.
微小RNA-500a通过转录后靶向BID促进肝细胞癌进展。
Cell Physiol Biochem. 2018;47(5):2046-2055. doi: 10.1159/000491472. Epub 2018 Jul 3.
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Biosci Rep. 2017 Dec 15;37(6). doi: 10.1042/BSR20170837. Print 2017 Dec 22.
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miR-500a-5p regulates oxidative stress response genes in breast cancer and predicts cancer survival.miR-500a-5p 调节乳腺癌中的氧化应激反应基因，并预测癌症的生存情况。
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