Liu Junnan, Dou Jianguo, Wang Wujiao, Liu Hengchuan, Qin Yunlang, Yang Qixin, Jiang Wencheng, Liang Yong, Liu Yuejiang, He Jiang, Mai Li, Li Ying, Wang Delin
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Department of Urology, The People's Hospital of Dazu, Chongqing 400016, P.R. China.
Oncol Lett. 2020 Mar;19(3):1815-1823. doi: 10.3892/ol.2020.11254. Epub 2020 Jan 7.
Citron kinase (CIT) is a Rho-effector protein kinase that is associated with several types of cancer. However, the role of CIT in prostate cancer (PCa) is unclear. The current study utilized microarray data obtained from The Cancer Genome Atlas, which was analyzed via Biometric Research Program array tools. Additionally, reverse transcription-quantitative (RT-q)PCR was performed to compare the mRNA expression of CIT in PCa tissue and in benign prostatic hyperplasia. The protein expression of CIT was detected in a consecutive cohort via immunochemistry and CIT was screened as a potential oncogene in PCa. The results of RT-qPCR demonstrated that the mRNA expression of CIT was increased in PCa tissues. Furthermore, immunochemistry revealed that CIT protein expression was positively associated with age at diagnosis, Gleason grade, serum PSA, clinical T stage, risk group, lymph node invasion and metastasis. When compared with the low expression group, patients with a high CIT expression exhibited shorter survival rates, cancer specific mortalities (CSM) and biochemical recurrence (BCR). In addition, multivariate analysis revealed that CIT was a potential predictor of CSM and BCR. The results revealed that CIT is overexpressed during the malignant progression of PCa and may be a predictor of a poor patient prognosis.
西特龙激酶（CIT）是一种与多种癌症相关的Rho效应蛋白激酶。然而，CIT在前列腺癌（PCa）中的作用尚不清楚。本研究利用从癌症基因组图谱获得的微阵列数据，通过生物统计学研究计划阵列工具进行分析。此外，进行逆转录定量（RT-q）PCR以比较CIT在PCa组织和良性前列腺增生中的mRNA表达。通过免疫化学在连续队列中检测CIT的蛋白表达，并将CIT筛选为PCa中的潜在癌基因。RT-qPCR结果表明，CIT的mRNA表达在PCa组织中增加。此外，免疫化学显示，CIT蛋白表达与诊断年龄、Gleason分级、血清PSA、临床T分期、风险组、淋巴结侵袭和转移呈正相关。与低表达组相比，CIT高表达的患者生存率、癌症特异性死亡率（CSM）和生化复发（BCR）较低。此外，多变量分析显示，CIT是CSM和BCR的潜在预测因子。结果表明，CIT在PCa的恶性进展过程中过表达，可能是患者预后不良的预测因子。
