Zhang Huan-Qing, Wang Hui-Ju, He Xiang-Lei, Ru Guo-Qing, Song Shu-Shu, Pan Hong-Ying, Zhang Cheng-Wu, Mou Xiao-Zhou, Hu Zhi-Ming
Department of Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital (People's Hospital of Hangzhou Medical College), Hangzhou 310014, China.
Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital (People's Hospital of Hangzhou Medical College), Hangzhou 310014, China.
Transl Cancer Res. 2020 Apr;9(4):2416-2423. doi: 10.21037/tcr.2020.03.58.
Citron kinase (CIT) is a protein related to cytokinesis and is an important abscission regulator. However, the relationship between CIT and hepatocellular carcinoma (HCC) is unclear. The aim of this study was to investigate the expression CIT in HCC tissues, and explore the connection between this expression and clinicopathological characteristics of HCC.
Immunohistochemistry staining on 235 HCC tissues and 96 non-tumorous liver tissues controls was performed to examine the CIT protein expression. We then analyzed the correlation between protein expression and clinicopathological parameters via χ tests, and we performed overall survival analyses via the Kaplan-Meier survival approach. Based on the online Oncomine Expression Array and UALCAN databases, we more broadly compared CIT mRNA expression between normal and HCC tissues. Finally, we compared CIT mRNA expression in these databases to protein expression in our study and explored potential sources for any observe differences.
Compared to normal tissues, CIT expression was significantly lower in HCC tissues. Low CIT expression was found to be related to gender, tumor size, Edmondson Grade, Microvascular invasion, serum AFP levels and poor overall survival. Based on the online databases, CIT mRNA expression was found to be high in HCC tissues and decreased in normal tissues. We hypothesize that this unexpected result is due to a negative feedback loop whereby low protein CIT levels mediate increased CIT mRNA levels.
Lower CIT protein levels are associated with a poorer prognosis in HCC patients, and lower CIT protein levels may mediate a negative feedback loop leading to increased CIT mRNA levels.
西特龙激酶（CIT）是一种与胞质分裂相关的蛋白质，是一种重要的分裂调节因子。然而，CIT与肝细胞癌（HCC）之间的关系尚不清楚。本研究的目的是调查CIT在HCC组织中的表达，并探讨这种表达与HCC临床病理特征之间的联系。
对235例HCC组织和96例非肿瘤性肝组织对照进行免疫组织化学染色，以检测CIT蛋白表达。然后通过χ检验分析蛋白表达与临床病理参数之间的相关性，并通过Kaplan-Meier生存法进行总生存分析。基于在线Oncomine表达阵列和UALCAN数据库，我们更广泛地比较了正常组织和HCC组织之间的CIT mRNA表达。最后，我们将这些数据库中的CIT mRNA表达与我们研究中的蛋白表达进行比较，并探讨观察到的差异的潜在来源。
与正常组织相比，HCC组织中CIT表达明显较低。发现低CIT表达与性别、肿瘤大小、Edmondson分级、微血管侵犯、血清AFP水平及总生存较差有关。基于在线数据库，发现HCC组织中CIT mRNA表达较高，而在正常组织中降低。我们推测这一意外结果是由于负反馈回路，即低水平的CIT蛋白介导了CIT mRNA水平的升高。
较低的CIT蛋白水平与HCC患者较差的预后相关，较低的CIT蛋白水平可能介导负反馈回路，导致CIT mRNA水平升高。
